椰子SSR标记的开发

Development and characterization of SSR markers from cocos nucifera

根据NCBI公布的椰子序列,挖掘SSR位点,共找到383个SSR位点,其中212个SSR位点能根据侧翼位点设计引物。采用28个SSR引物对10个椰子种质资源中进行扩增,发现10个标记具有较为丰富的多态性,同时评估了这10个椰子品种间的遗传距离,结果显示,我国与马来西亚椰子品种的平均遗传距离最大,而我国椰子品种内部的平均遗传距离最小;10个品种可被分为4类,其分组与地理来源相关。

Based on available sequence information of NCBI website, the paper exploited 338 SSR loci,and 212 loci could be designed according to flanking sequences. 28 primers were used to amplify the DNA of 10 coconut germplasm. The results showed that 10 markers have higher level of genetic diversity. Meanwhile, genetic distance between 10 coconut germplasm were evaluated, ranged from 0.63 between coconut germplasm form China and Malysia to 0.36 within coconut germplasm of China. Moreover, clustering analysis was also performed. The results showed that the 10 coconut varieties could be separated into 4 groups. The classification was closely correlation with geographic distribution of these coconut germplasm.