气相色谱法测定樟酚酊中樟脑含量

Determination of Camphor Content in Camphor Phenol Tincture by GC

目的建立樟酚酊中樟脑的含量测定方法。方法采用气相色谱(GC)法。以TM-Wax毛细管柱(30 mm×0.32 mm,0.25μm)为分析柱,FID检测器,分流进样,分流比40∶1,进样口温度为230℃,柱温为125℃,检测器温度为250℃,N2为载气。用水杨酸甲酯作内标物,以内标法定量。结果樟脑进样质量浓度在0.364 3～2.549 9 g/L范围内与樟脑和内标物的峰面积之比有良好线性关系,r=0.999 9,平均加样回收率为99.45%,RSD=0.79%。结论该方法灵敏、简便、结果准确、重现性好,可用于樟酚酊的质量控制。

Objective To establish a GC method for determining the camphor contents in Camphor Phenol Tincture. Methods The GC method was adopted with the TM-Wax capillary column （30 mm×0. 32 ram,0.25μm） as the analytical column,the FID detector,the streaming samples,the split ratio of 40： 1,the inlet temperature of 230 ℃ ,the column temperature of 125 ℃ ,the detector temperature of 250 ℃ and N2 as the cartier gas. Salicylic acid methyl ester was used as the internal standard substance with internal standard method for quantitation. Results The sample concentration of camphor in the range of 0. 364 3- 2. 549 9 g/L had good linear relation with the ratio of the peak areas of camphor and the internal substance, r = 0. 999 9. The average recovery rate of the sample was 99.45%, RSD =0. 79%. Conclusion This method is sensitive and simple with accurate detection Results and good reproducibility and can be used for the quality control of Camphor Phenol Tincturer.