短链线性聚丙烯酰胺-毛细管电泳分析DNA片段

The Determination of DNA Fragments by Use of Short Chain Linear Polyacrylamide Solution-capillary Electrophoresis

合成短链线性聚丙烯酰胺 ,解决了线性聚丙烯酰胺粘度大 ,难以填充毛细管的问题。在短链线性聚丙烯酰胺浓度为 4% ,电压 10 k V条件下 ,采用激光诱导荧光检测 (λex=488nm ,λem=5 13nm ) ,PCR Markers和p BR 32 2 DNA/ Hae Markers的 2

The linear polyacrylamide was used to modify the inner surface of capillary column. Short chain linear polyacrylamide(SLPA) was prepared and under the conditions of 4% short chain linear polyacrylamide, 7 kV and 37 cm×75 μm coated capillary column, the 28 fragments of the mixture of PCR and pBR 322 DNA/Hae Ⅲ Markers were well separated by use of short chain linear polyacrylamide solution capillary electrophoresis with laser induced fluorescent detector, of which the excitation wave length and emission wave length were 488 nm and 513 nm, respectively.