明胶/透明质酸复合支架材料的制备及性能

Preparation and characterization of Gel/HA composite scaffold

采用冷冻干燥方法,以1,2-乙基-3-(3-二甲基氨基丙基)-碳化二亚胺(EDC)为交联剂制备不同比例的明胶/透明质酸(Gel/HA)复合水凝胶。采用扫描电镜(SEM)和傅利叶红外光谱仪(FTIR)对所制备的明胶/透明质酸(Gel/HA)复合水凝胶进行表征,并研究复合水凝胶的亲水性能和溶胀行为。结果表明,随EDC浓度增大,水凝胶的表面和断面上的纤维丝减少,采用浓度为0.1%的EDC时,水凝胶表面和断面上纤维丝消失;红外光谱测试表明,加入EDC交联剂后的Gel/HA(5:5)水凝胶在1 710 cm-1处有明显的C=O的吸收峰,随EDC浓度增加,该吸收峰强度增加。水凝胶中随透明质酸比例的增加溶胀度增加、接触角减小。抗压强度测试显示,随EDC浓度增大,水凝胶的抗压强度提高,控制Gel和HA的混合比例可以调节水凝胶的抗压强度。在磷酸盐缓冲溶液(PBS)中加入溶菌酶可以有效提高水凝胶的降解速率。

The scaffolds, composing of gelatin （Gel） and hyaluronic acid （HA） with different proportions, were prepared by freeze-drying and chemical cross-linked by using 1-ethyl-3-（3-dimethylaminopropyl） carbodiimide hydrochloride （EDC） as a cross-linker. The microstructure and cross-linking of hydrogels were characterized by scaning electron microscopy （SEM） and Fourier transform infrared spectroscopy （FTIR）. The results showed the fliament was disappeared on the surface and cross-section of hydrogel with 0.1% EDC. In FTIR, a new peak at 1 710 cm^-1 was formed due to the existence of C=O, and the absorption peak became intensity with the increase of concentrations of EDC. The high swelling ratios and lower contact angle achieved for the scaffold with 100% hyaluronic acid. The compressive strength test showed that the compressive strength of the cross-linked hydrogel were much higher than those of the uncross-linked. The compressive strength of hydrogel was controled by adjusting the mixing ratio of Gel and HA. The mass loss rate of the hydrogel was accelerated by adding lysozyme into PBS.