Graves病患者外周血CD20、CD23表达的检测及意义

The Expression and Significance of CD23 and CD20 on Peripheral Blood from Patients with Graves′s Disease

目的:探讨Graves病(GD)患者外周血CD20+B、CD23+B和CD20+CD23+B淋巴细胞的表达情况及其意义。方法:收集GD患者89例,按病情分为GD初诊组36例、GD未缓解组13例和GD缓解组23例,17例健康对照者为对照组。应用流式细胞仪检测各组外周血CD20+B、CD23+B和CD20+CD23+B细胞的表达情况。结果:GD新发组患者外周血CD20+B、CD23+B和CD20+CD23+B细胞的比例分别为11.02±4.58%、18.78±5.71%、41.38±17.72%,明显高于缓解组6.60±2.86%、13.28±2.60%、22.94±13.46%和对照组6.12±2.55%、11.32±3.26%、27.05±13.84%,差异均有统计学意义(P<0.05);而CD23+B和CD20+CD23+B在GD治疗未缓解患者中的比例均明显高于GD治疗缓解组,差异有统计学意义(P<0.05),CD20+CD23+B表达变化与评价病情缓解情况的相关指标(FT3、FT4、TSH)有相关性(Spearman相关系数R分别为:0.510、0.426、-0.412,P<0.01)。结论:CD20+CD23+B表达的变化与GD患者病情进展及缓解密切相关,可作为GD治疗的一项新的监测指标。

Objective ：To investigate the expression levels of CD20 ＋ B, CD23 ＋ B and CD20 ＋ CD23 ＋ B lymphocytes on peripheral blood and access their significances in patients with Graves disease. Methods： The levels of CD20 ＋ , CD23 ＋ and CD20 ＋ CD23 ＋B lymphocytes on peripheral blood in 36 untreated GD patients, 13 GD patients not in remission, 23 GD patients in remission and 17 healthy controls were measured by using the flow cytometry. Results：The proportion of CD23 ＋ , CD20 ＋ and CD23 ＋ CD20 ＋ B lymphocytes on untreated GD patients 11.02 ＋ 4： 58%, 18.78 ＋ 5.71% ,41.38 ± 17.72% ,were higher notedly than that from GD patients not in remission 6. 60±2. 86%, 13. 28 ±2. 60% ,22. 94 ±13.46%, and the healthy controls 6. 12 ± 2. 55%, 11.32 ± 3.26% ,7. 05 ±13.84% （P 〈 0. 05 ）. The proportions of CD23 ＋ and CD23 ＋ CD20 ＋ B lymphocytes on GD patients not in remission were all higher than who were in remission （P 〈 0.05 ）. Besides, there were positive significant correlate between the outcomes and the proportion of CD23 ＋ CD20 ＋ B lymphocytes （ Spearman correlation coefficient R were 0. 0510,0. 426, - 0. 412, P 〈 0. 01 ）. Conclusions ： The expression of CD20 ＋ CD23 ＋ B lymphocytes on peripheral blood had relations with the development and the outcomes after treatment in the GD patients. So it maybe a new standard for monitoring the outcomes after treatment in the GD patients.