植物药生物碱94-95-10L对大鼠CSD模型脑内c-fos基因表达的影响

Effect of 94-95-10L on c-fos Gene Expression in Cortex of Rats with Cortical Spreading Depression

目的 :利用 KCl滴注大脑皮质制作大鼠皮层传播性抑制 (cortical spreading depression,CSD)模型 ,研究94- 95 - 10 L对 c- fos基因在大鼠脑额顶区内扣带回、新皮质和梨状区表达的影响。方法 :雄性 Wistar大鼠麻醉后 ,在左侧脑表面滴注 KCl(1mol/ L ,5μl)之前 5 0 m in,给予 94- 95 - 10 L (30 mg/ kg,ip.) ,氟桂嗪 (3m g/ kg,iv.)作为阳性对照。用免疫组化法观察 c- fos阳性细胞在以上 3区的表达。结果 :左侧脑表面滴注 KCl 12 0 min后 ,各区的 c- fos表达阳性细胞明显增多 ,而对侧基本无表达 ;尾静脉注射氟桂嗪能明显抑制 c- fos的表达 ;腹腔注射 94- 95 - 10 L使 c-fos表达明显减少。结论 :在由 KCl制造的 CSD模型中 ,94- 95 - 10 L能明显抑制 c- fos在额顶区的表达 ,说明该药对大鼠

Objective:Our aim was to study the effect of 94 95 10L on c fos gene expression in the cortex of rats with cortical spreading depression(CSD) induced by KCl. Methods: Wistar rats was administered 94 95 10 L (30 mg/kg,ip.), or flunarizine (3 mg/kg,iv.) 50 minutes before giving KCl (1 mol/L,5 μl) to the left cortex. Expression of c fos gene on frontoparietal cingulate, neocortex and piriform area(FrPa C, N, and P) was determined with immunohistochemical ABC method. Results: More c fos positive cells in the left cortex where KCl was administered than in the contracortex. Pretreatment with flunarizine reduced positive cells in FrPaC, N,P, and pretreatment with 94 95 10L attenuated the expression of c fos positive cells in the same areas. Conclusion: In the KCl induced CSD model, 94 95 10L significantly decreased the expression of c fos gene in FrPa C, N, P. Perhaps this drug can prevent aura of migraine and protect patients from migraine attack.