诱生型一氧化氮合酶在正常豚鼠耳蜗内的分布

Distribution of Inducible Nitric Oxide Synthase in Cochlea of Normal Guinea Pigs

目的 :观察诱生型一氧化氮合酶 (inducible nitric oxide synthase,i NOS)在正常豚鼠耳蜗内的分布。方法 :以特异性 i NOS抗体 ,应用免疫组化 ABC法结合显微图像定量分析技术 ,在 10只正常豚鼠耳蜗冰冻切片上检测 i NOS。结果 :i NOS的阳性免疫反应产物分布于耳蜗的血管纹、螺旋神经节细胞、Corti器的内、外毛细胞及神经纤维。结论 :正常豚鼠耳蜗有 i NOS表达 ,提示 i

Objective:This paper was to study the distribution of inducible nitric oxide synthase (iNOS) in cochlea of normal guinea pigs. Methods:The expression of iNOS was measured with ABC immunohistochemical staining combined with image quantitative analysis technique on frozen sections from cochlea of normal guinea pigs. Results: iNOS staining were observed in the stria vascularis, spiral ganglion cells, outer and inner hair cells of Corti's organ as well as nerve fibers in normal cochlea. Conclusion: iNOS was expressed under physiological conditions in cochlea of guinea pigs. This result suggested that iNOS may be involved in physiological functions in cochlea.