1型鸭疫里氏杆菌的分离鉴定及其ompA基因B细胞表位预测

Isolation and Identification of Riemerella anatipestifer Serotype 1 and Prediction of Epitopes on B Cell of ompA Gene

本研究从贵州发病鸭中分离到1株致病菌,经生化试验、16S rRNA序列分析、血清学试验和动物回归试验,鉴定为1型鸭疫里氏杆菌(RA),药敏试验结果表明,其对头孢拉定、头孢曲松钠、头孢噻肟和氟苯尼考高度敏感;同时根据GenBank中鸭疫里氏杆菌ompA基因序列,设计1对引物,成功克隆出鸭疫里氏杆菌ompA基因,扩增产物大小为1149 bp。采用DNAStar Protean程序,综合运用二级结构、亲水性、可塑性和抗原性指数等参数,对鸭疫里氏杆菌ompA基因的氨基酸序列进行了B细胞表位预测,为进一步研究鸭疫里氏杆菌表位疫苗和分子诊断技术的建立奠定基础。

One bacterium strain was isolated from incidence duck in Guizhou,tested by physiological and biochemical characteristics,16S rRNA gene sequencing,recursive infection experiment and antibiotic sensitivity as RA serotype 1.According to the gene sequences in GenBank of RA,one pairs of specific primer was designed for amplifying the specific fragments of ompA.The specific bands of 1149 bp were amplified.Using DNAStar Protean software,the amino acid sequence encoded by ompA gene of RA was analyzed in secondary structure,hydrophilicity,flexibility and antigenic indexes to predict B cell epitopes,which was to lay the foundation for further study of RA epitope vaccines and establishment of molecular diagnostic techniques.