高效液相色谱法测定大鼠肝微粒体温孵体系中血根碱的含量

Determination of Sanguinarine in Rat Liver Microsomes Incubation System by HPLC

本研究建立了大鼠肝微粒体温孵体系中血根碱的高效液相色谱含量测定方法。采用Shimadzu VP-ODS(150 mm×4.6 mm,5μm)为色谱柱,以乙腈-0.1%磷酸水溶液(30:70,V/V)为流动相,以1 mL/min流速进样量10μL在284 nm波长下进行检测,结果显示500和1000 nmol/L血根碱对照品溶液的方法回收率分别为98.3%和98.9%;250、500、1000 nmol/L标准溶液的日内RSD分别为3.76%、2.88%和3.56%,日间RSD分别为5.19%、4.96%和3.84%;血根碱的摩尔浓度在250～10000 nmol/L内与峰面积呈良好的线性关系,表明该方法简单、快速、灵敏度高、特异性好,可用于血根碱在肝微粒体温孵体系中的含量测定。

A HPLC method for the determination of sanguinarine in rat liver microsomes incubation system was developed.Chromatography was performed on a Shimadzu VP-ODS column（150 mm × 4.6 mm,5 μm）.An acetonitrile-0.1% phosphoric acid solution（V∶ V =30∶ 70） as the mobile phase was used on the wavelength of 284 nm.The recovery of 500 and 1000 nmol/L control solution was 98.3% and 98.9% respectively.The relative standard deviation of 250,500 and 1000 nmol/L standard solution for the within-day and between-day were 3.76%,2.88%,3.56% and 5.19%,4.96%,3.84%,respectively.The calibration curve was linear in the range from 250 to 10000 nmol/L with r = 0.9997.The method was accurate,sensitive and rapid for the determination of sanguinarine in rat microsomes incubation system.