犬瘟热病毒自然感染宠物犬不同部位检出率的分析

Canine Distemper Virus Naturally Infected Pet Dogs in Different Parts of the Detection Rate Analysis

本试验利用RT-PCR检测方法对犬瘟热病毒(CDV)自然感染宠物犬的不同部位样品进行检测,确定CDV在感染犬组织中的分布情况。对14只CDV阳性犬的血液、眼分泌物、鼻液、唾液、粪便和尿液进行检测,RT-PCR方法检出的阳性率分别为92.9%、85.7%、100%、85.7%、100%和66.7%,结果显示粪便和鼻液样品的检出率最高,检出率达100%,宜于作为检测对象取材。

Use of RT-PCR detection methods of canine distemper virus（CDV） natural infection pet dogs different parts of samples for testing,determine the distribution of CDV in the tissues of infected dogs.14 CDV positive dogs only to blood,eye secretions,nasal fluid,saliva,feces and urine testing,detected by RT-PCR positive rates were 92.9%,85.7%,100%,85.7%,100% and 66.7%, the results showed that the detection rate,the feces and nasal fluid samples was highest,was 100%,it is appropriate to the material as a detection object.