妇女降钙素受体基因型与骨密度的关系

Relationship between Calcitonin Receptor Genotypes and Bone Mineral Density

本文欲探讨中国汉族妇女降钙素受体（CTR）基因型频率分布及其与骨密度（BMD）的关系。对北京地区95名健康年轻妇女和127名绝经后妇女，采用聚合酶链反应-限制性片段长度多态性技术（PCR－RFLP）检测CTR基因型，同时通过PCR产物进行测序验证，应用双能X钱吸收法测量BMD值。发现汉族妇女CTR基因型频率分布依次为CC、TC、TT（分别占90．5％、8．6％、0．9％），等位基因频率C和T各为94．8％、5．2％。年轻妇女组TC基因型者在腰椎和股骨大转子部位的BMD值均高于CC基因型的相应部位（P＜0．05），绝经后妇女组不同基因型各部位BMD值无差别。提示汉族妇女CTR基因型频率分布不同于己报道的其他人种：CTR基因型与BMD间有一定关联。

To investigae the association of calcitonin receptor (CTR) genotypes with bone mineral density (BMD) inChinese Han ethnicity women. The CTR genotypes were determined by PCR-Restriction Fragment Length Polymorphism (RFLP) and sequence of PCR products in 95 healthy young women and 127 postmenopausal women of Hanethnicity in Beijing area. BMD was measured by duel-energy X-ray absorptiometry. The results showed that thefrequencies of CC, TC and TT were 90.5% 、 8.6% and 0.9% respectively, and the frequencies of allele C and Twere 94.8% and 5.2% respectively in Chinese women. TC genotype was higher BMD values in lumber and trochantercompared with CC genotype in young women (P<0.05). However, no association was observed between CTRgenotypes and BMD in postmenopausal women. The study suggested that the frequencies of CTR genotypes inChinese were different from those of other ethnicity, and there was some association between CTR genotypes andBMD in Chinese women.