服用低剂量醋酸棉酚对正常男性精子碱性核蛋白的影响

Effect of Low Dose Gossypol Treatment on Male Sperm Nuclear Basic Protein

目的 研究正常男性服用低剂量醋酸棉酚后对精子碱性核蛋白的影响。方法 志愿者 2 3例 ,其中对照组 8例 ,服药组 15例 ,服药组口服醋酸棉酚剂量为 15 mg/d,服药 12周达起效期后 ,改为 10 mg/d维持至 44周。于服药后 1、4、8、12、16、2 4、36、44周及停药后 10周采集精液 ,对照组于相同时相采取精液 ,分析精子碱性核蛋白的变化。结果 对照组总组蛋白 (TH)与总精核蛋白 (TP)的比值为 0 .17± 0 .0 7,服药组为 0 .6 3± 0 .79;对照组 P1型精核蛋白 (HP1)与 P2型精核蛋白 (HP2 + HP3)的比值为 1.0 5± 0 .2 1,服药组为 1.47± 0 .18;对照组与服药组两组参数相比较差异均有显著性 (P<0 .0 5 ,P<0 .0 1)。停药 10周后 ,服药组两组参数均恢复到正常状态。结论 服用低剂量醋酸棉酚能阻断组蛋白 -精核蛋白取代反应 (HPRR)及影响精核蛋白含量 ,这可能是醋酸棉酚导致不育的重要原因 ;低剂量醋酸棉酚导致的 HPRR及精核蛋白的改变是可逆的。

Study on human sperm nuclear basic proteins after low dose of gossypol treatment. Methods Twenty-three men with normal fertility were divided into two groups.The experimental group included 15 men which were treated daily with gossypol (15 mg/d for 12 weeks and treated with 10 mg/d for 32 weeks continously), 8 men were used in the control group. Semens were collected at 1, 4, 8, 12, 16, 24, 36 and 44 weeks after gossypol treatment and 10 weeks after stopping treatment. Total nuclear basic protein (TNBP) was extracted and TH/TP, HP1/(HP2+HP3) ratios were determined by scanning microdensitometry following electrophoresis of TNBP in polyacrylamide gels. Results The TH/TP ratio of control and the gossypol treated group were 0.17±0.07 and 0.63±0.79(P<0.05 );The HP1/(HP2+HP3) ratio of control and gossypol treated group were 1.05±0.21 and 1.47±0.18 (P<0.01). These two parameters were returned to normal after 10 weeks of stopping gossypol treatment. Conclusions The interruption of histone-to-protamine replacement reaction (HPRR) and alteration of nuclear basic proteins induced by low dose gossypol treatment might lead to infertility.Changes of HPRR and nuclear basic protein were reversible after stopping gossypol treatment.