5,7双羟色胺对不同功能运动神经元群调质CGRP配布式样的影响

Effect of 5,7-dihydroxytryptamine on the Distribution Pattern of Calcitonin Gene-related Peptide in Different Motoneuron Pools

目的 研究 5 ,7双羟色胺 (5 ,7- DHT)对不同功能运动神经元 (Mn)群调质 CGRP配布式样的影响。方法 利用霍乱毒素 B亚单位结合胶体金逆行标记神经元复合免疫细胞化学的非荧光双标记方法。结果 大鼠比目鱼肌 (慢肌 ) Mn(SOL - Mn)群和趾长伸肌 (快肌 ) Mn(EDL - Mn)群 CGRP配布式样不同 ,即 SOL - Mn群含较高比率 CGRP样免疫反应 (CGRP- L I)阴性和弱阳性 Mn,EDL - Mn群含较高比率 CGRP- L I强阳性 Mn;于脑室注射 5 ,7-DHT 14d后 ,可明显引起 SOL - Mn群 CGRP表达增加 ,所有标记的 SOL - Mn都呈 CGRP- L I阳性 ,CGRP- L I强阳性的 Mn比率显著增高 (P<0 .0 0 1) ;而 5 ,7- DHT则未引起 EDL - Mn群 CGRP- L I反应强度的增高。结论 调质CGRP在 SOL - Mn群和 EDL - Mn群的配布式样差异除与其所支配肌肉的功能活动方式密切相关外 ,还受到脊髓 5 -HT传入信息的影响 ,SOL - Mn群可能比 EDL - Mn群接受更多的 5 - HT能信息。

The effect of 5,7-dihydroxytryptamine(5,7-DHT)on distribution pattern of calcitonin gene-related peptide(CGRP) in different motoneuron pools was studied in rats. Methods Cholera toxin B subunit coupled with colloidal gold (CB-Au) retrograde labeling combined with CGRP immunocytochemistry technique was mainly used. Results In control group,there was a different distribution pattern of CGRP-LI content in soleus motoneuron (SOL-Mn)and extensor digitorum longus motoneuron (EDL-Mn)pools.SOL-Mn pools had a higher ratio of neurons lacking CGRP-LI/weak CGRP-LI and a lower proportion of strongly CGRP-LI labeled ones.Comparing with control,there was a marked shift to increasing of CGRP-LI content in SOL-Mn pool and slight decrease in EDL-Mn pools 14 days after 5,7-DHT.All the SOL-Mns were CGRP-LI positive and the ratio of CGRP-LI strongly positive Mns significantly increased (P<0.001)while 5,7-DHT did not increase the ratio of CGRP-LI strongly positive Mn in EDL-Mn pool. Conclusions Except muscular motor activity,the different distribution pattern of CGRP-LI content in SOL-Mn and EDL-Mn pools is related to different 5-HT inputs.SOL-Mns may receive more 5-HT inputs.