表皮生长因子受体对精子发生过程的影响

The Influence of Epidermal Growth Factor Receptor on Spermatogenesis

目的 探讨表皮生长因子受体 (EGFR)在精子发生过程的作用机制。 方法 ICR雄性小鼠在给予人绒毛膜促性腺激素 (HCG)和切除颌下腺前后采用链霉菌抗生物素蛋白 -过氧化酶 (SP)免疫组织化学结合体视学方法定量分析第 期生精小管上皮的 EGFR阳性静止期精母细胞和各级生精细胞。 结果 与假手术组相比 ,去颌下腺组 EGFR阳性反应静止期精母细胞明显减少 ,同时相应的生精小管的各级生精细胞也显著减少 (P<0 .0 5 )。与去颌下腺组相比 ,去颌下腺给药组和假手术给药组 EGFR阳性反应静止期精母细胞明显增多 ,同时相应的生精小管的生精细胞也显著增多 (P<0 .0 5 )。 结论 静止期精母细胞 EGFR表达减弱是切除颌下腺导致小鼠少精症的原因之一。 HCG可促进静止期精母细胞表达 EGFR。

Objective\ To investigate the receptor mechanism of epidermal growth factor(EGF) on spermatogenesis.\ Methods\ The epidermal growth factor receptor(EGFR) positive preleptotenes and various germ cells in stage Ⅶ of seminiferous epithelium of ICR mice before and after sialoadenectomy and human chorionic gonadotropin(HCG) injection were assayed quantitatively through methods of streptavidin\|peroxidase immunostaining and stereology.\ Results\ After sialoadenectomy,EGFR positively stained preleptotenes and various germ cells were significantly decreased(P<0.05).\ Compared with sialoadenectomized group, a significant increase in EGFR positively stained preleptotenes and various germ cells was observed in sialoadenectomized mice with HCG administration(P<0.05).\ Conclusion\ The weakening expression of EGFR in preleptotenes is one of the causes leading to oligospermia by sialoadenectomy.\ HCG can stimulate EGFR expression of preleptotenes to regulate spermatogenesis.\;