用血清药理学方法研究灵芝浸膏GLE的抗肿瘤作用机制

Study on the antitumor mechanism of Ganoderma lucidum Extract(GLE) by serologic pharmacological method

探讨灵芝浸膏 (GanodermalucidumExtract,GLE)的抗肿瘤作用及其机制。方法 :采用血清药理学与体内外抑瘤实验相结合方法。MTT法测肿瘤细胞增殖程度 ,流式细胞仪测细胞凋亡 ,同时采用生物法测TNF α,ELISA测定IFN γ ,并用RT PCR方法检测mRNA表达。结果 :(1)GLE(5、10、2 0 g·kg-1)灌胃显著抑制小鼠移植性肉瘤S180的生长 ;(2 )GLE直接加入S180细胞体外培养不能抑制其生长 ,亦无诱导其凋亡的作用 ;(3)GLE血清显著抑制S180细胞生长并诱导其凋亡 ,GLE血清中TNF α、INF γ水平显著升高 ,而且GLE显著促进其mRNA的表达。结论 :GLE无直接的抗肿瘤作用 ,其抗肿瘤作用是通过促进TNF α和INF γmRNA表达及其生成而实现的。

Objective: To study the antitumor activity and the mechanism of Ganoderma lucidum Extract (GLE) Methods: Serologic pharmacological method, and both in vivo and in vitro antitumor experiments were used in the studies. Proliferation of tumor cells was detected by MTT, TNF α was detected by biological assay, and INF γ by ELISA. The level of mRNA was detected with the method of RT PCR. Results: (1) GLE (5, 10, 20 g·kg -1 , i.g.) inhibited the growth of implanted sarcoma 180 in vivo significantly and dose dependently. (2) GLE directly adding to their cultured medium neither induced sarcoma 180 apoptosis nor restrained its proliferation in vitro . (3)GLE treated serum significantly induced sarcoma 180 apoptosis and inhibited its proliferation. The TNF α and INF γ raised in the cultured medium treated with GLE treated serum, GLE (5, 10, 20 g·kg -1 , i.g.) significantly increased TNF α and INF γ mRNA expression in mice. Conclusion: The anti tumor activity of GLE was derived from promoting mRNA expression of TNF α and INF γ, resulting in the production of TNF α and INF γ.