HPLC法测定制首乌及复方虫草胶囊中二苯乙烯苷的含量

Determination of 2,3,5,4′ tetrahydroxystilbene 2 O β D glucoside in Polygonum mutiflorum Thunb.and Fu Fang Chong Cao capsule by HPLC

建立原药材制首乌及复方虫草胶囊中 2 ,3 ,5 ,4′ 四羟基二苯乙烯 2 O β D 葡萄糖苷的含量测定方法。方法 :YWG C18柱 (4 .6mm× 2 5 0mm ,10 μm) ;流动相乙腈 水 (1∶4) ;检测波长 :32 0nm ,参比波长 380nm ;流速 :1ml·min-1;柱温 :室温。结果 :2 ,3 ,5 ,4′ 四羟基二苯乙烯 2 O β D 葡萄糖苷对照品在 1.6 5～ 3 .85 μg范围内 ,进样量与峰面积间呈良好的线性关系 ,r=0 .9997,平均回收率 (98.84± 0 .2 3) %。对照品在复方虫草胶囊中的峰纯度因子为 999.35 2。结论 :用HPLC法测定原药材制首乌及复方虫草胶囊中 2 ,3,5 ,4′ 四羟基葡萄糖苷的含量 ,操作简便 ,结果准确 ,可用于控制原药材制首乌及复方虫草胶囊制剂的质量。

Objective: To control the quality of raw medicinal material Polygonum mutiflorum Thunb. and Fu Fang Chong Cao capsule, and develop a method for the determination of 2,3,5,4′ tetrahydroxystilbene 2 O β D glucoside. Methods: HPLC method, YWG C18 column (4.6 mm×250 mm, 10 μm) with a mobile phase of acetonitrile water (1∶4); detecting wavelength at 320 nm, reference wavelength at 380 nm; flow rate :1 ml·min -1 ; column temperature: room temperature. Results: 2,3,5,4′ tetrahydroxystilbene 2 O β D glucoside was linear within the range of 1.65 3.85 μg, with a correlation coefficient of 0.999 7. The average recovery rate was (98.84±0.23)%. The purity factor of 2,3,5,4′ tetrahydroxystilbene 2 O β D glucoside in Fu Fang Chong Cao capsule was 999.352. Conclusion: The method is simple and accurate, and can be used for the quality control of raw medicinal material and Fu Fang Chong Cao capsule.