摘要
[目的]探讨禾长蠕孢菌(HGE)孢子及其代谢产物粗毒素对稗草防御酶系活性的影响。[方法]在室内条件下测定了禾长蠕孢菌孢子及其粗毒素对稗草防御酶系活性的影响。[结果]HGE菌孢子、粗毒素及二者混合处理稗草后,不同程度地影响了的稗草的PAL、POD的活性,对稗草SOD活性无明显影响,而且孢子和毒素对稗草防御酶系的影响具有相似性。[结论]由于病原真菌孢子和毒素对寄主的作用具有一定的相似性,基于病原菌的致病过程的复杂性,毒素可以替代病原菌研究其部分致病性作用机理。
[Objective] This study aimed to explore the effects of spores and crude toxins of Helminthosporium gramineum Rabenh f. sp. echinochloae(HGE) on the ac- tivity of defensive enzymes of barnyardgrass [Echinochloa crus-galli (L.) Beauv.]. [Method] The effects of spores and crude toxins of HGE, as well as the mixture of spores and crude toxins on the activity of defensive enzymes in barnyardgrass were determined under laboratory conditions. [Result] Spores and crude toxins of HGE had varying degrees of effects on PAL and POD activity, and no obvious effect on SOD activity in barnyardgrass. In addition, spores and toxins had some similar im- pacts on the defensive enzymes in barnyardgrass. [Conclusion] Since toxins have similar effects on the hosts as spores of fungal pathogen do, they can be a substi- tute for the fungal pathogen in studying the partial pathogenic mechanism of this pathogen due to its complexity in pathogenic process.
基金
Supported by Research Fund of the State Tobacco Monopoly Bureau(110201002002)
the Open Research Project of Key Laboratory of Tobacco Genetics and Breeding in the Tobacco Industry(TB201006)~~
关键词
禾长蠕孢菌
稗草
防御酶系
活性
Helminthosporium gramineum Rabenh f. sp. echinochloae (HGE)
barn- yardgrass[Echinochloa crus-galli(L.) Beauv.]
Defensive enzymes
Activity