大鼠Nrn1基因3’UTR克隆及序列分析

Cloning and sequence analysis of rat Nrn1 3'UTR

目的 neuritin(Nrn1/Cpg15)是一种神经突起生长因子,在神经修复过程中发挥着重要作用。本文旨在探讨miRNAs调控Nrn1基因表达的潜在性。方法采用PCR技术克隆大鼠Nrn1基因的3’UTR片段,同时利用MiRanda、DNAMAN软件对Nrn1基因序列及miRNA结合位点进行生物信息学分析。结果成功克隆了649bp的Nrn1 3’UTR核心区段;Nrn1基因在不同物种间的序列同源性呈高度保守。MiRanda靶标预测结果表明miR-204在3’UTR结合位点处高度保守,分值和能值较高,具有很强的潜在性。结论 Nrn1基因3’UTR在不同物种间序列保守性较高,miRanda软件预测miR-204结合位点处序列高度保守,进一步暗示Nrn1基因表达可能受到miR-204的调控。

Objective Neuritin（Nrn1/Cpg15）,which plays an important role in the process of neural restoration,is a neurite outgrowth factor.This study aims to investigate the potentiality of Nrn1 gene expression regulated by miRNAs.Methods The 3＇UTR of rat Nrn1 gene was cloned by PCR technology,and then bioinformatics analysis of gene sequence and miRNA binding sites were conducted by MiRanda and DNAMAN software.Results A 649 bp core sequence of Nrn1 gene 3＇UTR was amplified.The sequences of Nrn1 gene were highly conserved among different mammalian animals and human.The result of score and energy predicted by MiRanda showed that Nrn1 be the most potentially target gene of miR-204.Conclusion The 3＇UTR sequence of Nrn1 gene is conserved between different species,and the sequences of miR-204 binding sites are highly conserved among mammalian animals.It is further implied that the expression of Nrn1 gene is possibly regulated by miR-204.