不同组织来源单核/巨噬细胞对伴放线放线杆菌吞噬能力的比较研究

Comparative study of the phagocytosis of Actinobacillus actinomycetemcomitans by monocytes/macrophages of different tissues

目的:比较血液单核细胞(monocytes,Mo)、肺泡巨噬细胞(alveolar macrophage,AM)、腹腔巨噬细胞(peritoneal macrophage,PM)和肝脏枯否细胞(kuffer cells,KC)对伴放线放线杆菌(Actinobacillus actinomycetemcomitans,Aa)吞噬能力的差别。方法:3只健康新西兰兔,分离培养Mo、AM、PM和KC;荧光素标记Aa,以感染复数25∶1的比例感染细胞;流式细胞术检测0.5、1.0、1.5h时吞噬细菌的阳性细胞率和平均荧光强度,计算吞噬指数。结果:1.5h内,随着孵育时间增加,AM和PM对Aa吞噬能力逐渐升高,Mo和KC的吞噬能力在1h达到高峰;Mo、AM、PM和KC吞噬功能存在明显差异,孵育0.5h吞噬指数AM、PM>Mo、KC,1.0h时AM>PM>Mo、KC,1.5h时AM>PM>KC>Mo。结论:不同部位单核/巨噬细胞对Aa的吞噬能力不同,可能是造成牙周病对不同部位影响不同的重要原因。

Objective：To compare the phagocytosis ability of Actinobacillus actinomycetemcomitans （Aa） by peripheral mononuclear cells （ Mo）, alveolar macrophage （ AM）, peritoneal macrophage （ PM ） and kuffer cells （ KC ）. Methods ： Mo, AM, PM and KC from 3 healthy New Zealand rabbits were isolated and purificated. Aa was lableded with fluorescein isothiocyanate （FITC）. Monocytes/macrophages were infected with FITC-Aa in the ratio of 25：1 （25 bacteria： 1 cell）. The positive cell rates and mean fluorescent intensity （MFI） were detected by flow cytometry at 0.5,1. O, 1.5 h. Phagocytic index was calculated. Results： Within 1.5 h, the phagocytic indexes of AM,PM rose as the time increased; the phagocytosis ability of Mo and KC reached the peak at 1 h. Obvious difference of phagocytosis among Mo, AM, PM and KC was observed. At 0.5 h, the phagocytic indexes were ： AM and PM 〉 Mo, KC ; at 1.0 h, AM 〉 PM 〉 Mo and KC ; at 1.5 h, AM 〉 PM 〉 KC 〉 Mo. Conclusions ：The different phagocytosis of Aa by monocytes/macrophages of various tissues may be a important reason why periodontitis has different effects on different parts.