甲苯胺蓝活体染色对诊断尖锐湿疣的研究

The Diagnosis of Condyloma Acuminate with Intravital Staining with Toluidine Blue

目的:通过前瞻性研究,探索最经济、简单、快速、准确性高的诊断尖锐湿疣的方法。方法:对可疑生殖道尖锐湿疣的女性患者,采用甲苯胺蓝活体染色,刮片及阴道镜检查,然后取活检分别进行病理切片及PCR技术检测、对比。结果:甲苯胺蓝试验、刮片、阴道镜、病理组织学检查及PCR技术检测人乳头状瘤病毒感染,其湿疣阳性诊断率分别为:57.14%、37.86%、50.0%、60.71%、56.43%。甲苯胺蓝活体染色分别与病理组织学及PCR技术诊断尖锐湿疣结果进行对比,经统计学检验无显著性差异(P>0.05)。结论:甲苯胺蓝活体染色法是临床诊断尖锐湿疣经济、快速、简单易行,而且准确性较高的方法之一。

Purpose : A more economic,simple,rapid and accurate method for condyloma acuminate diagnosis was investigated by prospective study. Methods:Using intravital staining with toluidine blue, smear cytologic examination and vaginoscope,the female patients with condyloma acuminate were detected. Then,biopsy pathologic examination and PCR were performed for each case. Results:By intravital staining with toluidine blue, smear cytologic examination,vaginoscope,pathologic examina-tion and PCR, the positive rates of HPV were 57.14% , 37.86% , 50.0% , 60.71% and 56.43% , respectively. There was no statistic difference between intravital staining with toluidine blue and pathologic examination and PCR(P >0.05). Con-clusion: Intravital staining with toluidine blue is one of the most economic, rapid,simple and highly accurate method for clini-cal diagnosis of condyloma acuminate.