紫杉醇诱导Jurkat细胞凋亡及其机制探讨

Taxol-induced apoptosis in Jurkat T cell lymphoma cell line and its molecular mechanisms

目的 观察抗微管药物紫杉醇对T细胞淋巴瘤细胞株Jurkat是否具有凋亡诱导作用 ,并进一步研究bcl 2基因家族在此过程中的作用。方法 将不同浓度的紫杉醇作用于Jurkat细胞 ,观察其作用的时间效应及剂量效应 ;在光镜和电镜下观察其形态变化 ;用流式细胞术分析细胞DNA含量的改变并作DNA片段分析 ;用免疫组织化学及半定量逆转录 聚合酶链反应 (RT PCR)法观察在紫杉醇作用过程中凋亡调节基因bcl 2家族的mRNA和蛋白表达的变化。结果 紫杉醇能抑制Jurkat细胞生长 ,在一定剂量和时间范围内 ,主要引起细胞凋亡 ,并显示剂量和时间效应 ,在这个过程中bax转录及蛋白表达增加 ,并出现bcl xs的转录。结论 紫杉醇能特异地诱导Jurkat细胞凋亡 ,这为紫杉醇应用于T细胞淋巴瘤的治疗提供了依据 ,并为研究淋巴瘤细胞凋亡的基因调控提供了极好的模型 ,bax和bcl xs参与了紫杉醇诱导Jurkat细胞凋亡的基因调控。

Objective To observe whether antimicrotubular drug taxol can induce apoptosis in Jurkat T cell lymphoma cell line and the role of bcl 2 gene family in this process. Methods Different concentrations of taxol were used to treat Jurkat cells. Cell morphology was observed under light and electron microscope. Flow cytometry and electrophoresis were used to analyze DNA contents and DNA fragments.bcl 2 gene family proteins and mRNAs were studied by immunohistochemistry and semi quantitative RT PCR technique. Results Taxol could inhibit Jurkat cell growth. Within a certain range of treating time and dose, cells were induced apoptosis with a time and dose related manner. The expressions of bax protein and mRNA were increased and bcl x s mRNA became detectable after taxol treatment. Conclusion Taxol can specifically induce Jurkat cell apoptosis. It might provide a theoretical basis for clinical treatment and a good model for studying apoptotic gene modulation. Bax and bcl x s participate in the taxol induced apoptosis of Jurkat cells.