正交试验法优选淫羊藿配方颗粒的提取工艺

Optimization of Extraction Technology of Herba Epimedii Dispensing Granules by Orthogonal Experiment

目的:优选淫羊藿配方颗粒的提取工艺。方法:采用正交试验法,以总黄酮、淫羊藿苷的含量并结合干浸膏得率为考察指标,考察加水量、提取时间、提取次数3个因素,优选出淫羊藿配方颗粒的提取工艺。结果:淫羊藿配方颗粒的优选提取工艺为第1次加13倍量水,浸泡0.5 h,煎煮1 h,第2次加10倍量水,煎煮1 h。结论:该提取工艺能够有效地提取总黄酮、淫羊藿苷,为淫羊藿配方颗粒的标准化、规范化生产工艺提供依据。

This study was aimed to optimize the extraction process of Herba Epimedii dispensing granules. The extraction process of Herba Epimedii dispensing granules was optimized through orthogonal experiment while the content of general flavone and icariin. And the yield of dry extract was adopted as marks. And influences of solvent dosage, extraction time and extraction times were studies. The results showed that optimized extraction conditions of Herba Epimedii dispensing granules were determined as follows. The material was added 13 times amount of water, soaked for 0.5 h and extracted for 1 h at the first time. And for the second time, 10 times amount of water were added, and extracted for 1 h. It was concluded that the extraction process is efficacious for general flavone and icariin extraction. The method is suitable for the standardized production technology of Herba Epimedii dispensing granules.