地龙湿法超微粉碎与常规匀浆的“粉碎-溶出”动力学比较研究

Comparison of Earthworm Smash-dissolution Dynamics Using Conventional Homogenized and Wet Ultrafine Grinding Process

目的:对地龙湿法超微粉碎与常规匀浆两种提取工艺的提取效率进行比较。方法:采用粒径分析、扫描电镜、Bradford法测定蛋白含量等手段,建立"时间-粒径分布"与"时间-蛋白质累积溶出百分率"曲线,比较地龙粉碎与蛋白溶出的动力学过程。并采用HPLC指纹图谱技术,比较两种提取产物的成分差异。结果:地龙湿法超微粉碎与常规匀浆两种处理工艺所得成分的指纹图谱峰形基本一致,但前者所得色谱峰面积明显高于后者,表明前者提取效率明显高于后者。结论:湿法超微粉碎提取技术适用于地龙成分提取,整个工艺过程快速简捷,适于工业化推广。

This study was aimed to make a preliminary comparison of earthworm extraction efficiency using con- ventional homogenized and wet ultrafine grinding process, respectively. The curve of ＂time-particle size distribution＂ and ＂time-protein accumulation dissolution rate＂ was established through particle size analysis, scanning electron-microscopy and protein determination with Bradford method to make a comparison of earthworm smash- dissolution dynamics. And a comparison of extract composition with HPLC fingerprint was made. The results shows that the peak shapes of extracts from both processes were basically the same. While the composition peak area extracted with wet ultrafine grinding process were significantly higher than conventional homogenized process. It indicates that earthworm extracted efficiency using wet ultra.fine grinding process were obviously higher than conventional homogenized process. Wet ultrafine grinding can be used to extract earthworm compositions. The whole process is quick, simple and suitable for large-scale promotion.