牛磺酸改善染锰大鼠学习记忆能力的海马蛋白质组学研究

PROTEOMICS ANALYSIS FOR THE MOLECULAR MECHANISM OF TAURINE AGAINST IMPAIRED MEMORY INDUCED BY ACUTE MANGANESE ADMINISTRATION

目的通过双向电泳实验(2-dimensional gel electrophoresis,2-DE)研究牛磺酸减轻急性染锰诱导大鼠学习记忆损伤的分子机制。方法 60只SPF雄性SD大鼠随机分3组:生理盐水(NS)对照组,连续腹膜内注射(ip)0.9%NS;染锰组,每日ip MnCl2.4H2O(15mg/kg.d);牛磺酸预防组,染锰的同时ip牛磺酸(200 mg/kg.d),各组大鼠连续注射4w。最后一次干预结束后进行水迷宫实验观察大鼠学习记忆能力变化,分离大鼠海马组织并制备总蛋白,2-DE分离差异蛋白质并经辅助激光解析电离飞行时间质谱仪(MALDI-TOF-MS)分析和Mascot数据库检索鉴定差异蛋白。结果水迷宫实验显示:平均逃避潜伏时间(s)染锰组大鼠39.8±2.3,较对照组29.5±2.5及牛磺酸预防组29.4±2.3显著延长(P<0.05),平台搜索次数各组间无显著差异,染锰组与对照组、预防组比对共有10个差异表达的蛋白质斑点,鉴定出4个表达下调的蛋白质,分别是NADH dehydrogenase(ubiquinone)Fe-S protein 1、Galactokinase I、whaq protein、beta-centractin。结论牛磺酸对急性染锰诱导的大鼠空间学习能力损伤有明显改善作用,蛋白质组学研究初步鉴定出4个在三组中共存的表达下调蛋白质点。[营养学报,2012,34(6):553-557]

Objective To investigate the molecular mechanism of taurine against impaired study and memory capacity induced by acute MnCl2 administration.Method Sixty SPF male rats were randomly divided into 3 groups.Control group was peritoneally injected saline everyday for 4 w.MnCl2-treated group was injected i.p.15 mg/（kg.d） MnCl2 for 4 w,Taurine-prevented group was injected i.p.both MnCl2 and 200 mg/kg.d taurine together for 4 w.Rats were trained to proceed Morris water maze to determine study and memory capacity.Hippocampus was separated to prepare for 2-DE.The variant spots were collected and identified by MALDI-TOF-MS and Mascot software.Results The average escape latency of rats in MnCl2-treated group was（39.8±2.3 s）,markedly longer than those in control（29.5±2.5s）,and taurine-prevented（29.4±2.3s） group（P0.05）.But the probing times were not significantly different among different groups.4 of 10 common variant spots from 3 groups were identified.There were NADH dehydrogenase（ubiquinone） Fe-S protein 1,galactokinase I,whaq protein,beta-centractin.Conclusion Taurine improves impaired study and memory capacity induced by MnCl2.Proteomics analysis reveals that 4 down-regulated spots may be responsible for taurine action against MnCl2 neurotoxicity.[ACTA NUTRIMENTA SINICA,2012,34（6）：553-557]