三羟异黄酮与葡萄糖醛酸内酯解毒保肝的比较研究

COMPARATIVE STUDY ON DETOXIFING AND PROTECTIVE EFFECTS OF GENISTEIN AND GLUCUROLATONE AGAINST LIVER INJURY

目的比较三羟异黄酮(Gen)与药物葡萄糖醛酸内酯(肝泰乐,Glu)对乙酰氨基酚(扑热息痛,APAP)诱导小鼠急性肝损伤的解毒保肝效果。方法实验小鼠分成正常对照(control)、APAP模型(model)、Gen、Glu及联合组(GG)共五组,灌胃后分别测定各组小鼠血清丙氨酸转氨酶(ALT)、天冬氨酸转移酶(AST)水平,以及总蛋白(TP)、白蛋白(ALB)、血清及肝脏还原性谷胱甘肽(GSH)并观察肝组织切片。结果以APAP模型组为基准,Gen组小鼠血清AST、ALT活性降低幅度分别比Glu组高61.9%和7.6%(P<0.01),小鼠血清TP、ALB、GSH含量升高幅度分别比Glu组高3.20%、9.89%和25.25%(P<0.01),肝组织病理学检查表明GG组及Gen组肝细胞AgNORs染色颗粒数量及银染核仁面积/细胞核面积(IS)值均显著高于Glu组(P<0.05)。结论 Gen与Glu保肝解毒机制不同,前者效果优于后者,其机制可能与诱导肝细胞DNA转录活性有关。[营养学报,2012,34(6):595-598]

Objective To observe the different effects of genistein（Gen） and glucurolatone（Glu） on hepatitis induced by acetaminophen（APAP）.Method Thirty-five mice were randomly divided into 5 groups： normal control（NC）,acetaminophen treatment（model）,Gen,Glu and combination（GG） group,and were administered with different doses of Gen or Glu for 5 d.All groups were administered 300 mg/kg APAP except normal control in the sixth day and all mice were sacrificed 24 h later.Alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,total protein（TP）,albumin（ALB） and glutathione（GSH） in serum or liver were measured and the pathological changes of liver tissue were observed.Results Taken biochemical indicators of APAP group as basal value,the decrements of AST and ALT in Gen group were more than in Glu group by 61.9% and 7.6% respectively（P 0.01）,while the increments of TP,ALB and GSH in Gen group evidently more than in Glu group by 3.20%,9.89% and 25.25%（P 0.01）respectively.The pathologic section showed that the number of AgNORs granules and IS（Index of staining） values in hepatic cells of Gen and GG groups were significantly higher than those in Glu group（P0.05）.Conclusion Gen is better than Glu and different in hepatic protection mechanisms which may relate to DNA transcription activity.[ACTA NUTRIMENTA SINICA,2012,34（6）：595-598]