鼻咽癌组织中miR-21的表达变化及意义

Expression and clinical significance of miR-21 in nasopharyngeal carcinoma

目的观察鼻咽癌组织中miR-21的表达变化,并探讨其临床意义。方法收集54例鼻咽癌患者活检组织标本(观察组),35例正常及慢性鼻咽炎黏膜组织标本(对照组),采用实时荧光定量PCR(qRT-PCR)检测两组miR-21的表达,分析miR-21表达水平与鼻咽癌临床病理参数及与患者预后关系。结果观察组中miR-21平均表达量为(16.76±1.73)×10-3,对照组为(2.39±0.62)×10-3,两组比较,P<0.01。临床Ⅲ/Ⅳ期鼻咽癌miR-21表达水平明显高于临床Ⅰ/Ⅱ期鼻咽癌组织(P<0.05);有淋巴结转移的鼻咽癌组织明显高于无转移的鼻咽癌(P<0.01);Kaplan-Meier生存分析发现,miR-21表达越高患者生存率越低(P<0.05)。结论鼻咽癌组织中miR-21的表达明显升高,与鼻咽癌的发生、临床进展及预后密切相关,可能是鼻咽癌新的诊断预后分子标志物及潜在的治疗靶点。

Objective To investigate the expression and clinical significance of the miR-21 in tissue of nasopharyngeal carcinoma（NPC）.Methods Biopsies of 54 cases of NPC（observed group） and 35 control tissues of normal and inflammatory nasopharyngeal cases（control group）were collected,and qRT-PCR was used to detect the miR-21 expression in those specimens.The correlation between the miR-21 expression and clinicopathologic parameter of NPC,prognosis were analyzed.Results The average expression level of miR-21 in observed group and control group was（16.76±1.73）×10-3 and（2.39±0.62）×10-3,which showed significant difference（P0.01）.Moreover,the expression of miR-21 was higher in NPC stage Ⅲ/Ⅳ than that in stageⅠ/Ⅱ（P0.05）;and tissue with lymph node metastasis was higher than that without lymph node metastasis（P0.01）.By using Kaplan-Meier survival curves,NPC patients with relatively high expression of miR-21 showed higher mortality compared with low expression of miR-21.Conclusions The expression of miR-21 increases in tissue of NPC,which is associated with the carcinogenesis,progression,and prognosis of nasopharyngeal carcinoma and it can be used as a new biomarker of diagnosis and prognosis and a potential target of therapy and in NPC.