PUMA与Caspase-3在乳腺癌组织中的表达及临床意义

Expression and clinical significance of PUMA and Caspase-3 in breast carcinoma

目的:探讨乳腺癌组织中p53正向细胞凋亡调控因子(PUMA)和Caspase-3的表达及临床意义。方法:采用免疫组织化学方法检测65例浸润性乳腺癌、25例乳腺纤维腺瘤及20例乳腺癌旁组织中PUMA和Caspase-3的表达情况。结果:PUMA在浸润性乳腺癌组织中的表达明显低于乳腺纤维腺瘤组织(15.4%vs 36.0%,P<0.05),乳腺纤维腺瘤组织中的表达明显低于乳腺癌旁组织(36.0%vs70.0%,P<0.05)。PUMA的表达与肿瘤淋巴结转移、TNM分期、术后复发、雌激素受体(ER)及人类表皮生长因子受体-2(HER-2)密切相关(P<0.05)。Caspase-3在乳腺癌组织中的表达明显低于乳腺纤维腺瘤组织(21.5%vs 44.0%,P<0.05),乳腺纤维腺瘤组织中的表达明显低于乳腺癌旁组织(44.0%vs80.0%,P<0.05)。Caspase-3的表达与淋巴结转移、术后复发、ER、HER-2及肿瘤分化程度密切相关(P<0.05)。PUMA与Caspase-3在浸润性乳腺癌组织中的表达呈正相关(r=0.491,P<0.05)。结论:PUMA和Caspase-3在浸润性乳腺癌的发生、发展中起着重要作用,可能成为乳腺癌的分子标志物或治疗的新靶点。

Objective： To study the expression and clinical significance of PUMA and Caspase-3 in breast carcinoma. Methods： Immunochemical method was performed to detect the ex- pression of PUMA and Caspase-3 in 65 cases of breast carcinoma, 25 cases of fibroadenoma and 20 cases of cancer surrounding tissues. Results： Expression of PUMA in breast cancinoma tissue （15.4%） was significantly lower than that in fibroadenoma （36.0%, P〈0.05）. The Latter was signifi- cantly lower than that in cancer surrounding tissues （70.0%, P〈0.05）. The expression of PUMA was closely related to lymph node metastasis, TNM stages, postoperative recurrence, estrogen receptor and HER-2 receptor （P〈0.05）. Expression of Caspase-3 in breast cancinoma tissue （21.5%） was significantly lower than that in fibroadenoma （44.0%, P〈0.05）. The Latter was significantly lower than that in cancer surrounding tissues （80.0%, P〈0.05）. Caspase-3 was closely related to lymph node metastasis, postoperative recurrence, estrogen receptor, HER-2 receptor and pathological dif- ferentiation （P〈0.05）. The expression of PUMA was positive correlated with Caspase-3（P〈0.05）. Conclusion： PUMA and Caspase-3 might play an important role in occurence and development of breast carcinorma which perhapes became molecule marker or new therapeutic target.