Th1／Th2细胞相关因子对肝癌家族聚集性的影响研究

Exploration of the relationship between the expression level of Th1/Th2 cytokines and the familial aggregatioh of the hepatocellular carcinoma

目的探讨机体Th1／Th2细胞相关因子及免疫状态在肝癌发生及家族聚集性中的作用。方法以IFN-1和IL-2代表Th1类细胞因子，IL-4和IL-10代表Th2类细胞因子，利用酶联免疫吸附试验检测95对经配对设计的肝癌高发家族成员和无癌家族成员外周血IFN-γ、IL-2、IL4、IL-10的水平。结果肝癌高发家族成员中肝癌患者的Th1细胞因子水平显著高于无癌家族成员，Th2细胞因子水平显著低于无癌家族成员（P〈0．05）；肝癌高发家族中未患肝癌的家族成员，Th1／Th2细胞相关因子与无癌家族成员相比无明显差异（P〉0．05）。结论肝癌高发家族中的肝癌患者存在Th1／Th2平衡失调，向Th2方向漂移，抗乙型肝炎病毒（HBV）感染及抗肿瘤免疫功能低下导致HBV长期慢性感染，进而引起肝癌，在HBV感染家族聚集的基础上发生肝癌的家族聚集。肝癌高发家族中未患肝癌的成员Th1／Th2细胞因子水平无明显变化。推测肝癌的免疫功能异常是肝细胞癌变后逐渐形成的，而不是癌变前存在的免疫异常导致了癌变。

Objective To investigate the effect of Th1/Th2 cytokines and immune state on the oc- currence and familial aggregation of hepatocellular carcinoma（HCC）. Methods Ninety-five members whose families have had two or even more HCC patients （high-occurrence families） were selected as the case group, by matching with the same nationality, gender, residential area, age±5 years old, 95 members whose families had no any cancer were selected as the control. The level of peripheral blood Th1 type cytokines such as in- terferon-γ （ IFN-γ）, interleukin-2 （ IL-2 ） and Th2 type cytokines such as interleukin-4 （ IL-4 ）, interleukin-10 （IL-IO） were detected by enzyme linked immunosorbent assay（ELISA）. Results There was a Th1/Th2 se- rum cytokine imbalance profile in members of HCC high-occurrence family. The levels of IFN-γ, and IL-2 were significantly lower in members of HCC high-occurrence family than that of the controls. The levels of IL- 4 and IL - 1 0 were higher in members of HCC high - occurrence family than that of the controls. Conclusion There was a poor cellular immune state in members of HCC in the high-occurrence families. Th1 type cyto- kines was inhibited, and Th2 type cytokines was enhanced, so more susceptible to HBV chronic infection. It might be the mechanism of HCC occurrence and familial aggregation.