摘要
目的探讨机体Th1/Th2细胞相关因子及免疫状态在肝癌发生及家族聚集性中的作用。方法以IFN-1和IL-2代表Th1类细胞因子,IL-4和IL-10代表Th2类细胞因子,利用酶联免疫吸附试验检测95对经配对设计的肝癌高发家族成员和无癌家族成员外周血IFN-γ、IL-2、IL4、IL-10的水平。结果肝癌高发家族成员中肝癌患者的Th1细胞因子水平显著高于无癌家族成员,Th2细胞因子水平显著低于无癌家族成员(P〈0.05);肝癌高发家族中未患肝癌的家族成员,Th1/Th2细胞相关因子与无癌家族成员相比无明显差异(P〉0.05)。结论肝癌高发家族中的肝癌患者存在Th1/Th2平衡失调,向Th2方向漂移,抗乙型肝炎病毒(HBV)感染及抗肿瘤免疫功能低下导致HBV长期慢性感染,进而引起肝癌,在HBV感染家族聚集的基础上发生肝癌的家族聚集。肝癌高发家族中未患肝癌的成员Th1/Th2细胞因子水平无明显变化。推测肝癌的免疫功能异常是肝细胞癌变后逐渐形成的,而不是癌变前存在的免疫异常导致了癌变。
Objective To investigate the effect of Th1/Th2 cytokines and immune state on the oc- currence and familial aggregation of hepatocellular carcinoma(HCC). Methods Ninety-five members whose families have had two or even more HCC patients (high-occurrence families) were selected as the case group, by matching with the same nationality, gender, residential area, age±5 years old, 95 members whose families had no any cancer were selected as the control. The level of peripheral blood Th1 type cytokines such as in- terferon-γ ( IFN-γ), interleukin-2 ( IL-2 ) and Th2 type cytokines such as interleukin-4 ( IL-4 ), interleukin-10 (IL-IO) were detected by enzyme linked immunosorbent assay(ELISA). Results There was a Th1/Th2 se- rum cytokine imbalance profile in members of HCC high-occurrence family. The levels of IFN-γ, and IL-2 were significantly lower in members of HCC high-occurrence family than that of the controls. The levels of IL- 4 and IL - 1 0 were higher in members of HCC high - occurrence family than that of the controls. Conclusion There was a poor cellular immune state in members of HCC in the high-occurrence families. Th1 type cyto- kines was inhibited, and Th2 type cytokines was enhanced, so more susceptible to HBV chronic infection. It might be the mechanism of HCC occurrence and familial aggregation.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2012年第11期1000-1004,共5页
Chinese Journal of Microbiology and Immunology
基金
广西自然科学基金(桂科自2010GXNSFA013185)
广西卫生厅重点科研项目(桂卫重2010012)
国家自然科学基金(30960170)
关键词
肝细胞癌
细胞因子
免疫状态
家族聚集
Hepatocellular carcinoma
Cytokines
Immune state
Familial aggregation