CSPG4基因在鼻咽癌中的表达分析

CSPG4 Gene Expression in Nasopharyngeal Carcinoma and Control Tissues

目的:研究硫酸软骨素多糖蛋白4(CSPG4)基因在鼻咽癌及对照组织中的表达。方法:采用实时荧光定量PCR测定CSPG4基因在4例正常鼻咽上皮和18例鼻咽癌组织(其中Ⅱ期6例,Ⅲ期5例,Ⅳ期7例)中的表达情况;通过构建包含92例对照和187例鼻咽癌的组织芯片,应用免疫组织化学检测CSPG4蛋白的表达和分布。结果:正常鼻咽上皮中CSPG4基因mRNA表达水平较低,在鼻咽癌中CSPG4表达上调且与临床分期呈正相关,差异具有统计学意义(P=0.017)。免疫组织化学结果表明鼻咽癌中CSPG4蛋白表达水平高于对照组,91.98%的鼻咽癌组织中CSPG4为强阳性表达,而对照组的强阳性率为76.09%(P=0.001)结论:CSPG4在鼻咽癌中随着临床进展表达逐渐上调,可能参与了鼻咽癌的发生发展,具体机制及临床应用前景值得进一步深入研究。

Objective： To study the expression of chondroitin sulfate proteoglycan 4 （CSPG4） gene in nasopharyngeal carcinoma （NPC） and control tissues. Methods： The CSPG4 mRNA expression ofgene in 4 cases with normal nasopharyngeal epithelial tissue and 18 cases with NPC, including 6 stage- II, 5 stage- III, and 7 stage- IV cases, was determined by real-time quantitative PCR. A tissue microarray containing 92 cases of control and 187 cases of NPC tissues was constructed, and the CSPG4 protein expression level was assayed by immunohistochemistry. Results： CSPG4 mRNA was barely expressed in the normal tissues of nasopharyngeal epithelium, but significantly upregulated in the NPC biopsies （P=0.017） and positively correlated with the NPC clinical stage. The CSPG4 protein expression was also higher in NPC than in the controls. CSPG4 was significantly expressed in 91.98% of NPC cases, whereas the percentage of high CSPG4 expression cases was 76.09% in the control group （P=0.001）. Conclusion： The CSPG4 gene is upregulated in NPC biopsies and is directly associated with NPC clinical staging, implying the probable involvement of CSPG4 in the development of NPC. The detailed mechanism of CSPG4 in carcinogenesis and its potential clinical application in NPC should be investigated further.