人原发性肝细胞癌中锌指蛋白A20、NF-κB p65及多药耐药P-gp蛋白的表达

Expressions of Zinc Finger Protein A20,NF-κB p65 Protein,and P-glycoprotein in Primary Hepatocellular Carcinoma

目的检测锌指蛋白A20、NF-κB p65蛋白及P-gp蛋白在人原发性肝细胞癌和癌旁组织中的表达,比较两者之间的差异,并探讨其与肝细胞癌临床病理学特征的关系。方法收集2009年2月至2010年8月期间于华西医院肝脏及血管外科行手术切除的32例原发性肝细胞癌患者的肝癌组织标本及对应的癌旁组织26例,行免疫组织化学染色,同时收集患者完整的临床病理学资料。结果锌指蛋白A20、NF-κB p65蛋白及P-gp蛋白在原发性肝细胞癌组织中的表达阳性率分别为87.5%(28/32)、81.3%(26/32)及65.6%(21/32),高于癌旁组织中的61.5%(16/26)、34.6%(9/26)及30.8%(8/26),P<0.05。锌指蛋白A20的表达与乙肝表面抗原(HbsAg)表达情况及肝硬变情况有关(P<0.05);NF-κB p65蛋白和P-gp蛋白的表达均与HbsAg表达情况有关(P<0.05)。结论锌指蛋白A20、NF-κB p65蛋白及P-gp蛋白在原发性肝细胞癌组织中均呈高表达,而在癌旁组织中呈低表达;3种蛋白可能对肝脏肿瘤的良恶性判断起指导作用。

Objective To compare the positive rate of zinc finger protein A20,NF-κB p65 protein,and P-glyco-protein between primary hepatocellular carcinoma（HCC） tissues and paratumor tissues,and to explore the relationship between the 3 kinds of proteins and pathological features of HCC.Methods Thirty-two HCC tissues and 26 paratumor tissues resected from patients with HCC treated in our hospital from Feb.2009 to Aug.2010 were enrolled.Clinical data were also collected from files.The expressions of zinc finger protein A20,NF-κB p65 protein,and P-glycoprotein were tested by immunohistochemistry.Results The positive rate of zinc finger protein A20,NF-κB p65 protein,and P-glycoprotein in HCC tissues were 87.5%（28/32）,81.3%（26/32）,and 65.6%（21/32）,respectively,which were higher than that in paratumor tissues（61.5%（16/26）,34.6%（9/26）,and 30.8%（8/26）,respectively）,P0.05.The three kinds of proteins were all closely related with HbsAg,and zinc finger protein A20 was related with cirrhosis in addition（P0.05）.Conclusion sThe positive rate of zinc protein A20,NF-κB p65 protein,and P-glycoprotein are much higher in primary HCC tissues than that in paratumor tissus,and they may play an important role in preoperative determination of hepatic tumors.