MCM-41固载牛血清白蛋白复合材料的制备、表征及发光

Preparation,Characterization and Luminescence of MCM-41 Immobilized Bovine Serum Albumin Composite Material

本研究首先利用水热法制备出了纳米分子筛MCM-41,然后把牛血清白蛋白(Bovine serum albumin,BSA)物理吸附法固载在主体MCM-41中,制备出(MCM-41)-BSA复合材料。化学分析表明,BSA已进入上述主体材料中,固载量为55.68 mg(BSA)/g(MCM-41)。粉末X射线衍射(XRD)结果表明,复合材料的结晶度保持良好,分子筛的基本骨架保存完好没有由于BSA的引入而遭到破坏。77 K低温N2吸附-解吸附的研究结果说明,BSA部分进入了分子筛孔道中。扫描电镜(SEM)测量结果显示,(MCM-41)-BSA样品的直径为100±10 nm。发光研究表明,(MCM-41)-BSA样品中BSA的构象未发生变化。

In this research,nanoscale MCM-41 molecular sieve was prepared using hydrothermal method and then bovine serum albumin（BSA） was immobilized in the above host to prepare（MCM-41）-BSA composite materials by physical adsorption method.Chemical analysis showed that BSA had entered into the above-stated host material and its immobilization amount was 55.68 mg（BSA）/g（MCM-41）.Powder X-ray diffraction（XRD） results indicated that crystallinity degree of composite materials was maintained good and basic frameworks of the molecular sieve were preserved intact and were not destructed due to BSA introduction.The low temperature N2 adsorption-desorption study results at 77 K showed that BSA had already partially entered in the molecular sieve pore channels.The measurement results by scanning electron microscopy（SEM） displayed that the diameter of（MCM-41）-BSA sample was 100 ±10 nm.The luminescence study showed that in the（MCM-41）-BSA sample the conformation of BSA was not changed.