IL-22和IL-17 mRNA在慢性HBV感染者PBMCs中的表达及其意义

IL-22 and IL-17 mRNA expression in PBMCs from patients with chronic hepatitis B virus infection

目的:探讨慢性HBV感染者外周血单个核细胞IL-22和IL-17 mRNA表达及其对病情转归的影响.方法:以慢性乙型肝炎中度患者33例、重度患者21例、重型肝炎患者16例、乙型肝炎肝硬化患者16例和健康对照10例为研究对象,采用RT-PCR方法检测患者外周血单个核细胞IL-22和IL-17 mRNA的表达.结果:CHB中度、CHB重度和肝硬化患者IL-22表达水平无统计学意义,但均高于健康对照组,差异有统计学意义(P=0.000);而重型肝炎患者IL-22表达水平低于该3组患者,高于健康对照组,但差异无统计学意义(P=0.064).各组IL-17表达水平相似但均高于健康对照组,差异有统计学意义(P=0.000);重型肝炎患者IL-17表达水平均低于其他3组患者,与CHB中度患者比较差异有统计学意义(P=0.014),与CHB重度和肝硬化患者比较差异无统计学意义(P=0.172,0.968).结论:IL-22的下调不利于肝细胞损伤后的修复,IL-22分泌的增加对减轻肝组织的损伤特别是重型肝炎的恢复可能是有意义的.慢性HBV感染患者IL-17均明显升高,提示其可能参与慢性HBV感染肝组织炎症的发生,且对慢性肝病纤维化起一定作用.

AIM： To detect the expression of IL-22 and IL-17 mRNAs in peripheral blood mononuclear cells （PBMCs） from patients with chronic hepatitis B （CHB） infection and to analyze their significance in the pathogenesis of CHB. METHODS： Thirty-three patients with moderate CHB, 21 patients with severe CHB, 16 patients with liver failure, and 16 cirrhotic patients were enrolled in the study. Ten healthy volunteers were used as controls. RT-PCR method was used to detect the expression of IL-22 and IL-17 mRNAs in PBMCs from these subjects. RESULTS： IL-22 expression in PBMCs from pat- ients with moderate CHB, severe CHB, or cir-rhosis was comparable, but all was higher than that from controls （P = 0.000）. IL-22 expression in PBMCs from patients with liver failure was significantly lower than that from other three groups of patients （P = 0.000）. IL-17 expression in PBMCs from four groups of patients was higher than that from controls （P=0.000）. IL-17 expression in PBMCs from patients with liver failure was slightly lower than that from other three groups of patients, although no statistical difference was found. CONCLUSION： Down-regulation of IL-22 is unfavorable to repair of hepatic cells after liver injury. Up-regulation of IL-22 expression may be important in alleviating liver damage. IL-17 levels in patients infected with HBV are in- creased significantly. IL-17 may participate in the inflammatory process in chronic HBV infec- tion and play an important role in development of liver fibrosis.