Piwil2基因在肝癌组织中mRNA及蛋白的表达

Clinical signif icance of expression of Piwil2 in hepatocellular carcinoma

目的:检测肝癌组织中Piwil2基因mRNA和蛋白的表达,进而探讨Piwil2基因在临床诊断中的意义.方法:采用反转录-聚合酶链反应(RT-PCR)和Western blot印迹技术,检测了70例肝癌及其对应癌旁组织中PiWil2基因mRNA及其蛋白的表达并结合临床资料进行分析.结果:PiWil2 mRNA在肝癌中的表达量相对值高于癌旁的肝脏组织的表达量(0.91±0.04vs0.32±0.04,P<0.05).Piwil2基因mRNA的表达与患者的肝内转移、分化程度及癌栓等病理因素方面的差异有统计学意义(P<0.05).蛋白在肝癌中的表达均高于对应的癌旁组织.Piwil2的蛋白质表达与患者的肝内转移及癌栓等病理因素方面的差异有统计学意义(P<0.05).Piwil2基因mRNA的表达与Piwil2的蛋白质的表达有相关性(P<0.05).结论:Piwil2有可能成为肝癌的检测及治疗的一种分子标志物.

AIM： To investigate the mRNA and protein ex- pression of Piwil2 in hepatocellular carcinoma （HCC）, and to analyze their clinical significance. METHODS： Reverse transcription-polymerase chain reaction （RT-PCR） and Western blot were used to detect the expression of Piwil2 mRNA and protein in 70 surgical HCC specimens and matched tumor-adjacent non-cancerous speci- mens. RESULTS： The relative expression level of Pi- wil2 mRNA was significantly higher in HCC than in tumor-adjacent non-cancerous tissue （0.91±0.04 vs 0.32±0.04, t = 37.240, P 〈 0.05）. Piwil2 mRNA expression in HCC was correlated with tumor differentiation, intrahepatic invasion and metastasis. The protein expression of Piwil2 in HCC was also significantly higher than that in tumor-adjacent non-cancerous tissues. Piwil2 protein expression in HCC was correlated with intrahepatic invasion and metastasis. There was a significant correlation between the mR_NA and protein expression of Piwil2 （x^2 = 13.113, P 〈 0.01）.CONCLUSION： Piwil2 may be used as a novel biomarker for detection and therapy of HCC.