分泌性中耳炎中白细胞介素-10的测定意义

Role of interleukin 10 in otitis media with effusion

目的探讨白细胞介素-10(interleukin 10,IL-10)在成人分泌性中耳炎(otitis media with effusion,OME)患者中耳积液及外周血中的表达水平,及其在OME发病中的可能作用机制。方法采用双抗体夹心酶联免疫吸附试验法检测36例(45耳)成人OME患者中耳积液、外周血浆及30例正常人外周血浆中IL-10的表达水平。结果 IL-10在中耳积液中的含量显著高于同组患者外周血浆中含量(P<0.01),而后者与正常人血浆中IL-10含量比较,差异无统计学意义(P>0.05);慢性OME患者中耳积液及外周血浆中IL-10含量显著高于急性OME组(P<0.01,P<0.05);慢性OME患者中耳积液中IL-10的含量显著高于同组患者血浆中含量(P<0.01),而后者与正常人血浆中IL-10含量比较,差异无统计学意义(P>0.05);接受2次及以上鼓膜穿刺/置管OME患者中耳积液中IL-10含量高于首次鼓膜穿刺/置管OME患者(P<0.05),同组患者血浆中IL-10的含量在以上2组患者间比较,差异无统计学意义(P>0.05);黏液性中耳积液中IL-10的含量高于浆液性中耳积液(P<0.05)。结论 IL-10作为重要的免疫介质可能与成人OME的慢性持续状态有关。

Objective This research was designed to study the role of interleukin 10（IL-10） in the pathogenesis of otitis media with effusion（OME） in adults.Methods The expression of IL-10 in middle ear effusion（MEE） and blood plasma were measured in 36 adults（45 ears） with OME by means of enzyme linked immunosorbent assay（ELISA）,as well as in 30 normal volunteers.Results The expression of IL-10 in MEEs was significantly higher than that in the plasma of OME patients（P〈0.01）,while the latter had no statistically significant difference with the plasma of normal volunteers（P〉0.05）.The expression of IL-10 in MEEs of chronic course was significantly higher than those MEEs of acute course.While the IL-10 concentration in patients＇ plasma was concerned,the plasma with chronic OME was higher than that in acute OME（P〈0.01,P〈0.05）;in chronic OME,expression of IL-10 in MEEs was higher than those in plasma（P〈0.01）,while there was no significant difference between plasma of patients with chronic OME and normal plasma（P〈0.05）;The expression of IL-10 in MEEs of patients who had been treated with myringotomy or/and insertion of ventilation tube more than once were higher than those MEEs treated for the first time,respectively（P〈0.05）.As far as the IL-10 expression in patients＇ plasma was concerned,there was no significant difference between those treated more than once and those treated firstly（P〈0.05）.The expression of IL-10 was higher in mucoid MEEs than in serous MEEs（P〈0.05）.Conclusion IL-10 as an important immunoregulatory mediators,may play an important role in chronic course of OME in adults.