人羊膜上皮细胞移植对脑缺血缺氧新生大鼠脑生长相关蛋白43、神经颗粒素及神经功能恢复的影响

Effects of tansplantation of human amniotic epithelial cells on GAP-43, neurogranin expression and neurological recovery in neonatal rats with hypoxic-ischemic brain encephalopathy

目的：探讨人羊膜上皮细胞移植对缺氧缺血新生大鼠海马生长相关蛋白43（GAP-43）、神经颗粒素（Ng）的表达及神经功能恢复的影响。方法：7d龄大鼠随机分为假手术组、模型组和移植组，移植组和模型组经侧脑室注入人羊膜上皮细胞悬液和PBS治疗；假手术组不治疗。移植后观察大鼠神经行为变化，免疫组织化学检测脑BrdU阳性细胞、GAP-43、Ng的表达。结果：移植组大鼠的神经功能较缺血缺氧组明显改善，移植后第3周脑切片检出BrdU阳性细胞存在。GAP-43和Ng在第3周的表达，模型组均低于假手术组，移植组较模型组增加。移植后第3周的神经评分和斜坡实验耗时与相应模型组相比明显减少。结论：人羊膜上皮细胞可减轻缺氧缺血后神经元的损伤，GAP-43和Ng蛋白表达增强，促进神经轴突的生长与延伸，从而改善神经功能。

Objective： To study the expression of growth associated protein 43 （GAP-43）, neurogranin （Ng） and neurologi- cal recovery after transplantation of human amniotic epithelial cells （hAECs） into the brain of neonatal rats with hypoxic-is chemic injury. Methods： 7-day-old rats were randomly divided into a sham operation control, a model group and a transplan- tation group. The hAECs marked with BrdU and PBS were respectively injected into the cerebral ventricle of the transplan- tation group and model group, and not into the sham operation group. Neurological changes were observed after transplanta- tion. BrdU-positive cells and the expression of GAP-43 and Ng proteins were detected by immunohistochemistry after trans- plantation. Results： Compared with the model group, the neurological function and time consumption of Pitch experiment was sig- nificantly improved in the transplantation group; BrdU positive ceils were detected in the transplantation group at 3 weeks following transplantation; GAP-43 and Ng proteins in the model group were significantly lower than those in the sham operation group, and those in the transplantation group were higher than those in the model group at 3 weeks following transplantation. Conclusion： hAECs injected into the cerebral ventricles could reduce hypoxic-ischemic injury to neurons, and utrregulate growth associated pro- tein 43 and neurogranin expression, which promotes axonal growth and extension and finally improves neurological function.