慢性日本血吸虫病患者唾液中特异性抗体的检测

DETECTION OF SPECIFIC ANTIBODIES IN SALIVA OF PATIENTS WITH CHRONIC SCHISTOSOMIASIS JAPONICA

[目的 ]研究日本血吸虫病患者唾液中抗血吸虫特异性抗体检测的可行性。 [方法 ]应用 EL ISA检测血吸虫病患者及正常人唾液中的抗血吸虫抗体并与血清中的特异性抗体比较。[结果 ]慢性血吸虫病患者唾液 32份 ,阳性 2 9份 (90 .6 % ) ,血清 32份 ,阳性 32份 (10 0 % ) ,经χ2 检验两者间差别无显著性意义 (P>0 .0 5 )。正常人唾液140份 ,阳性 8份 (5 .75 % ) ,正常人血清 15 6份 ,阳性 6份 (3.8% ) ,两者间差别无显著性意义 (P>0 .0 5 )。 [结论

Objective] To investigate the feasibility of detecting specific antibodies in the saliva of schistosomiasis patients. [Methods] Specific antibodies in saliva samples of 32 schistosomiasis patients and 140 normal individuals were detected by using ELISA and the results were compared with those detected for specific antibodies in serum. [Results] The sensitivity and specificity of the ELISA were 90 6% and 94 4%, respectively, being slightly lower than that of the 100% sensitivity and 96 2% specificity for the serum. No significant difference between them was found ( P >0 05). [Conclusion] Detecting specific antibodies in saliva can be used for immunodiagnosis of schistosomiasis japonica as a noninvasive method in field surveys.