蒿甲醚对日本血吸虫糖代谢酶的影响

EFFECT OF ARTEMETHER ON ENZYMES INVOLVED IN CARBOHYDRATE METABOLISM OF SCHISTOSOMA JAPONICUM *

[目的 ]观察蒿甲醚 (Art)对日本血吸虫糖代谢酶活性的影响。 [方法 ]小鼠感染日本血吸虫尾蚴 4～ 5wk后 ,1次灌服 Art30 0 mg/ kg,于治疗后 2 4h、48h或 72 h剖杀收集雌虫和雄虫 ,制备虫匀浆上清液 ,然后进行平板淀粉凝胶电泳 ,以对 9种糖代谢酶活性作定性观察。 [结果 ]经 Art作用 2 4～ 48h后 ,雌虫的己糖激酶、苹果酸脱氢酶、苹果酸酶和磷酸葡萄糖异构酶活性均有所下降 ;72 h后 ,上述 4种酶以及醛缩酶、6 -磷酸葡萄糖脱氢酶、6 -磷酸葡糖酸脱氢酶和 6 -磷酸甘露糖异构酶的活性均明显降低 ,甚至完全被抑制。在上述观察时间内 ,雄虫的醛缩酶、6 -磷酸甘露糖异构酶和 6 -磷酸葡萄糖脱氢酶活性受一定的影响。 [结论

Objective To study the effect of artemether on several enzymes involved in carbohydrate metabolism of Schistosoma japonicum . [Methods] Mice infected with Schistosoma japonicum cercariae for 4～5 weeks were administered intragastrically with artemether 300 mg/kg and killed 24～72 h after medication. The supernatant fluids of female and male worm homogenates were prepared for determining 9 essential enzymes of carbohydrate metabolism by using horizontal starch gel electrophoresis. [Results] The activities of 8 out of 9 enzymes (i.e. hexokinase, aldolase, glucosephophate isomerase, malate dehydrogenase, malic enzyme, glucose 6 phosphate dehydrogenase, 6 phosphogluconate dehydrogenase and mannose 6 phosphate isomerase) in female worms from artemether treated mice were obviously inhibited 24～72 h after treatment. In male worms, only aldolase , mannose 6 phosphate isomerase and glucose 6 phosphate dehydrogenase were slightly inhibited. [Conclusion] Artemether displayed apparent effects on the carbohydrate metabolism of female schistosomes.