角质细胞生长因子对高氧暴露新生大鼠肺表面活性蛋白B表达的影响

Effect of Keratinocyte Growth Factor on the Expression of Surfactant Protein B in Hyperoxia-Exposed Newborn Rats

目的:研究角质细胞生长因子(KGF)对高氧暴露下新生大鼠肺组织表面活性蛋白B(SP-B)表达的影响。方法:将新生的108只SD大鼠随机分为空气组、高氧组和KGF干预组,每组36只,每组分为3、7、14d 3个亚组。高氧组、KGF干预组大鼠持续暴露于氧体积分数>950 mL/L氧箱中,KGF干预组于吸氧同时在背部皮下注射重组人角质细胞生长因子(rhKGF)1 mg/d,连用3d后改为0.5 mg/d至实验结束。空气组和高氧组给予等量生理盐水背部皮下注射。空气组大鼠呼吸空气。3、7、14d亚组分别于3、7、14d取大鼠肺组织,采用免疫组化方法检测肺组织中SP-B表达的积分光密度值(OD)。结果:高氧组3d SP-B表达强度较空气组增强(P<0.05),高氧组7d SP-B表达强度与空气组比较,差异无统计学意义(P>0.05),高氧组14d SP-B表达强度较空气组和KGF干预组减弱,差异有统计学意义(P<0.01)。空气组和KGF干预组SP-B在3、7、14d的表达比较差异无统计学意义(P>0.05)。结论:长时间暴露于高氧环境,可导致SP-B表达减弱或功能障碍,是高氧肺损伤的重要原因;KGF能促进肺表面活性物质合成,对高氧性肺损伤具有重要的保护作用。

Objective： To explore the effect of Keratinocyte Growth Factor（KGF） on expression of SP-B in hyperoxia-exposed newborn rats＇ lung tissue.Methods： One hundred and eight newborn SD rats were randomly divided into an air group,a hyperoxia group and a KGF intervention group.Each group had 36 rats.The rats in each group were randomly tested on the 3rd,7th,14th day respectively.Rats in hyperoxia group and KGF intervention group were continually exposed to more than 950 mL·L-1 of the oxygen until the end of the experiment.KGF intervention group simultaneously undertook oxygen inhalation,hypodermic injection of 1 mg·d-1 rhKGF on the back at the first 3 days and 0.5 mg·d-1 three days later until the end of the experiment.Air group and hyperoxia group were offered an equivalent amount of NS.The rats in air group took air.The sub-groups of the 3rd,7th and 14th day had lung tissue removed in the corresponding times,and the expression of SP-B protein was detected by immunohistochemistry.Results： SP-B expression of hyperoxia group on the 3rd day was more than that of air group（P0.05）.There was no significant difference on the 7th day between hyperoxia group and air group on SP-B（P0.05）.There was a significant difference on the 14th day between hyperoxia group and air group,KGF intervention group on SP-B（P0.01）.Compared with KGF group,SP-B expression of air group had no significant difference on the 3rd,7th,14th days.Conclusions： The longtime hyperoxia exposure leads to a down regulation or functional impairment of the SP-B expression,which may be an important factor that influences the development of hyperoxia lung injury.KGF may increase pulmonary surfactant secretion,and play a protective role in hyperoxia-induced lung injury.