人组织工程软骨的实验研究

Study of human tissue engineering cartilage

目的 了解组织工程研究的基本原理 ,完善制作组织工程化人软骨的技术方法 ,探讨天然可吸收材料组织 ,引导再生胶原膜作为人软骨细胞体外培养支架的可行性 ,为广泛开展组织工程的研究与应用开辟新的途径。方法 利用组织工程技术制作人组织工程化软骨 ,并应用组织学方法 ,对获得的人组织工程化组织形态和功能进行表征。结果 培养的软骨细胞均匀沉降于医用组织引导再生胶原膜上 ,1周后形成一层乳白色软骨样组织 ;植入 8周后从裸鼠体内获得软骨样组织 ,组织学检测证实软骨细胞的存在 ,软骨细胞可以分泌硫酸软骨素。结论 医用组织引导再生胶原膜以其特有的三维结构和细胞网结功能 ,可以作为组织工程技术应用研究中软骨细胞培养的支架 ;利用组织工程技术 ,能够完成人组织工程化软骨的制作。

Objective\ To investigate the technique of human tissue\|engineered cartilage and to study the medical collagen membrane of guided tissue regeneration (GTR) as the carrier of in vitro chondrocytes culture.Methods\ Tissue engineering technique was used to make human Tissue\|engineered cartilage,which was examined histologically and functionally.Results\ It was found that the chondrocytes seeded on the medical collagen membrane of GTR grew well.A layer of milk white and cartilage\|like tissue grew on the surface of medical collagen membrane of GTR after l week.It was demonstrated that the cartilage\|like tissue was strong enough to be transferred after being implanted for 8 weeks.The cartilage\|like tissue was proved to be human tissue engineered cartilage by HE stain and Alcian blue\|poncean S stain.The chondrocytes could secrete chondroitin sulfate as proved by Lev\|Spicer stain.Conclusions\ The medical collagen membrane of GTR has characteristics of three\|dimensional structure and cell reticular function,and it has the possibility to be developed as a natural scaffold for tissue engineering,The results indicate that it is possible to make human tissue\|engineered cartilage with tissue\|engineering technique. \;