生存素和PEDF蛋白在食管癌中的表达及临床意义

Expressions of Survivin and PEDF protein in esophageal neoplasms tissue and its clinical significance

目的探讨生存素(Survivin)和色素上皮衍生因子(PEDF)在食管癌组织中的表达及其临床意义。方法利用链霉素抗生物素蛋白-过氧化物酶免疫组织化学法检测86例食管癌组织、6例正常组织及6例炎症组织中Survivin和PEDF蛋白的表达。结果食管癌组织中Survivin和PEDF蛋白的阳性表达率均显著高于正常组织(P<0.05)。食管癌中Survivin与PEDF蛋白的表达与肿瘤TNM分期、分化程度及淋巴结转移有关,而与患者的年龄、性别无关。86例食管癌组织中Survivin和PEDF蛋白的表达呈显著负相关(r=-0.353,P=0.003)。结论 Survivin与PEDF蛋白在肿瘤的生成中具有拮抗作用,二者同时检测对判断食管癌的恶性程度及其预后具有重要意义。

Objective To explore expression of Survivin and pigment epithelium-derived factor（PEDF） in esophageal carcinoma tissue and its clinical significance. Methods Streptavidin-perosidase immunohistochemistry method was employed to detect Survivin and PEDF protein expression in 86 cases of esophageal carcinoma tissue,6 cases of normal and inflammation tissue. Results The positive rates of Survivin and PEDF protein expression in esophageal carcinoma tissue were significant higher than those in normal tissue（P^0.05）. Survivin and PEDF protein expression in esophageal carcinoma tissue were related to TNM staging, tumor differ- entiation and lymph node metastasis,and showed no correlation with age and gender of patients. There was a negative correlation between Survivin and PEDF protein expression in 86 cases of esophageal carcinoma tissue（r=-0. 353, P= 0. 003）. Conclusion Survivin and PEDF protein have antagonistic role in tumor formation,and their co-detection can be of great importance for estima tion of malignant degree and prognosis of esophageal carcinoma.