急性脑梗塞血瘀证与一氧化氮、内皮素相关性研究

Study on Relationship of Parameters of Nitric Oxide, Endothelin and Blood Stasis Syndrome in Traditional Chinese Medicine in Acute Cerebral Infarction

目的：探讨急性脑梗塞（ａｃｕｔｅ ｃｅｒｅｂｒａｌ ｉｎｆａｒｃｔｉｏｎ， ＡＣＩ）患者血浆一氧化氮（ｎｉｔｒｉｃ ｏｘｉｄｅ， ＮＯ）、内皮素（ｅｎｄｏｔｈｅｌｉｎ， ＥＴ）与中医血瘀证的关系。方法：７３例ＡＣＩ患者分为血瘀证组５８例（其中重度１８例，中度１９例，轻度２１例）和非血瘀证组１５例，并与３０名健康体检者作对照，测定血浆ＮＯ和ＥＴ含量。结果：血瘀证组、ＡＣＩ组ＥＴ、ＥＴ／ＮＯ均显著高于健康对照组（Ｐ＜０．０１）；血瘀证组轻、中、重度组ＥＴ、ＥＴ／ＮＯ依次增高，重度组与轻、中度组比较有显著性差异（Ｐ＜０．０），中度组与轻度组比较仅ＥＴ有显著性差异（Ｐ＜０．０１）；ＡＣＩ组、血瘀证组、非血瘀证组ＮＯ含量与健康对照组比较均显著降低（Ｐ＜０．０５，Ｐ＜０．０１）；血瘀证轻、中、重度各组间ＮＯ含量比较无显著性差异（Ｐ＞ ０．０５）。结论：ＮＯ和ＥＴ参与了ＡＣＩ发展过程，推测由于血管及神经细胞等分泌ＮＯ、ＥＴ的功能失衡可能是血瘀证产生的病理基础。

To explore the relationship of parameters of nitric oxide (NO), endothelin (ET) and blood stasis syndrome (BSS) of traditional Chinese medicine (TCM) in acute cerebral infarction (ACI). Methods: Seventy-three patients of ACI (ACI group) were divided into two groups by the score of BSS: BSS group (included 18 cases of serious BSS, 19 cases of moderate BSS, and 21 cases of mild BSS); and non-BSS group (15 cases); while 30 healthy subjects were taken as the control group, their NO and ET were determined. Results: Compared with the control group, the level of ET and ratio of ET/NO raised significantly in the ACI group and the BSS group (P < 0. 01 ). The levels of ET and ratio of ET/NO of mild, moderate and serious BSS increased in order, but only the serious BSS in comparing with mild, moderate group of BSS had significant difference (P < 0. 01). The NO levels of ACI group, non-BSS group in comparing with healthy Control, there was significant decrease (P < 0. 05, P < 0. 01 ). The NO concentration among the mild, moderate and serious group of BSS had insignificant difference (P > 0. 05 ). Conclusion: NO and ET participates the formation and development of ACI, owing to the disturbed NO and ET secretion by vascular, nerve cells etc., these might be the pathologic basis of BSS occurrence.