补肾益智方对Alzheimer病模型大鼠脑内生长抑素及其mRNA表达阳性神经元的影响

Effects of Bushen Yizhi Recipe on Somatostatin-like Immunopositive and Somatostatin Messenger Ribonucleic Acid Expressed-Positive Neurons in Alzheimer's Disease Model Rats

目的：观察补肾益智方对Ｄ－半乳糖加速衰老加上脑Ｍｅｙｎｅｒｔ核损毁Ａｌｚｈｅｉｍｅｒ病（ＡＤ）模型大鼠脑内生长抑素（ｓｏｍａｔｏｓｔａｔｉｎ， ＳＳ）的影响。方法：将１５月龄初老年雌性Ｗｉｓｔａｒ大鼠随机分为正常对照组、ＡＤ模型组、双益平对照组和补肾益智方观察组。模型组大鼠以Ｄ－半乳糖腹腔注射加上鹅膏蕈氨酸（ｉ－ｂｏｔｅｎｉｃ ａｃｉｄ，ＩＢＯ）脑内Ｍｅｙｎｅｒｔ核注射造模，双益平对照组和补肾益智方观察组分别在造模的同时灌胃双益平和补肾益智方。运用免疫组织化学和ｃＲＮＡ探针原位杂交方法检测大鼠大脑顶叶皮质、海马 ＣＡ_１和齿状回门区ＳＳ免疫和ＳＳｍＲＮＡ表达阳性神经元数目。结果：补肾益智方组大鼠脑顶叶、海马ＣＡ_１、齿状回门区ＳＳ免疫和ＳＳｍＲＮＡ表达阳性神经元的数目及其积分光密度较模型组有增加趋势，部分组间比较有显著性差异。结论：补肾益智方对Ｄ－半乳糖加上ＩＢＯ损毁Ｍｅｙｎｅｒｔ核ＡＤ模型大鼠脑内ＳＳ能神经元有一定程度的保护作用。

To observe the effect of Bushen Yizhi Recipe (BYD) on somatostatin-like (SS-like) immunoreactivity and SS mRNA expressed-positive neurons in Alzheimer disease (AD) model rats induced by D-galactose intraperitoneal and ibotenic acid (IBO) intracerebral injection. Methods: Fifteen months Wistar rats were randomly divided into 4 groups: normal control; AD model control induced by D-galactose intraperitoneal injection and lesion of basal nucleus of Meynert with IBO; Huperzine A (Hup-A) treated group and BYD treated group fed with Hup-A or BYD respectively. The SS-like immunoreactivity and SS mRNA expressed-positive neurons in parietal lobe, hippocampal CAI field and hilus of the dentate gyrus in the brains of rats, which were detected with immunohistochemistry and DIG-labelling cRNA-probe in situ hybridization. Results: Counts and integrations of optic density of SS-like immuno-reactivity and SS-mRNA expressed neurons in parietal lobe, hippocampal CAI field and hilus of the dentate gyrus in BYD treated group increased compared with AD model rats. Conclusion: BYD might protect the SS-ergic system against impairment of D-galactose and IBO in the brains of the AD model rats.