三氧化二砷诱导HL-60细胞凋亡的实验研究

Experimental Study on Apoptosis of HL-60 Cell Induced by Arsenic Trioxide

目的：探讨三氧化二砷（Ａｓ_２Ｏ_３）治疗白血病的疗效机理。方法：以 ＨＬ－６０细胞株为体外模型，终浓度为０．２０μｍｏｌ／Ｌ Ａｓ２_Ｏ_３作用２４、４８ｈ后，用ＰＩ／ＡｎｎｅｘｉｎＶ－ＦＩＴＣ双标记，流式细胞术观察Ａｓ_２Ｏ_３对ＨＬ－６０细胞凋亡及其对细胞株端粒酶活性的影响。结果：Ａｓ２_Ｏ_３作用于ＨＬ－６０细胞后ＰＩ阴性／ＡｎｎｅｘｉｎＶ－ＦＩＴＣ阳性细胞增多，提示细胞发生凋亡，ＤＮＡ周期分析显示主要作用在细胞Ｇ_２－Ｍ期，４８ｈ对端粒酶有轻微抑制作用。结论：Ａｓ_２Ｏ_３主要通过诱导细胞凋亡杀伤白血病细胞。

To explore the mechanism of arsenic (arsenic trioxide, As_2O_3) in treating leukemia. Methods: The in vitro effect of arsenic trioxide on HL-60 model double-labelled with PI/AnnexinV-FITC was studied with flow cytometry. Function on telomerase activity was also observed. Results: Alter the effect of arsenic on HL60 cell line, PI negative/ AnnexinV- FITC positive cells increased, it mainly acts at t he G2 - M phase that suggests the apoptosis takes place,. mild inhibition on telomerase was also found 48 hours later. Conclusion: Apoptosis induced by arsenic trioxide was the main mechanism in killing or inhibiting leukemic cells.