中国野生华东葡萄芪合成酶基因启动子转化番茄的研究

Research on the STS Gene Promoter of Chinese Wild Vitis pseudoreticulata Transformed Tomato

中国野生华东葡萄"白河-35-1"中的芪合成酶基因启动子被证明具有病原菌和胁迫诱导表达活性。现将芪合成酶基因置于增添不同增强子的该启动子下,构建4个植物表达载体,通过农杆菌介导法进行了转化番茄的研究,并对转基因株系抗番茄灰霉病表现及其白藜芦醇含量变化进行了测定。结果表明:经PCR-Southern鉴定,获得转基因株系4个共54株;不同载体转化的番茄株系对灰霉病抗性均高于野生型,但抗性存在差异;添加增强元件enhancer启动子启动的芪合成酶基因表达的番茄株系,可使白藜芦醇含量提高4.4倍。

Stilbene synthase gene（STS）and its specific promoter which was related to disease resistance and stress were previously isolated from Chinese wild Vitis pseudoreticulataaccession‘Baihe-35-1’.In this paper,the promoter was added different enhancement components for construction of three enhanced expression vectors.The results showed that four lines including 54transgenic tomato plants which were tested by PCR-Southern were obtained by Agrobacteriummediated genetic transformation.The disease resistance index of transgenic tomato plants was measured after inoculation of Botrytis cinerea,and the resistance of all transgenic plants was increased to some degree.Resveratrol content analysis showed that addition of enhancerelement could increase the amount of resveratrol expression to 4.4times.