CatWalk步态分析系统评价构建膀胱反射弧对下肢功能的影响

CatWalk gait analysis in assessment of functional changes of the hind paw after SD rats beingestablished the artificial bladder reflex arc

目的通过CatWalk步态分析系统评价离断k脊神经根对下肢功能的影响，以明确将其用于构建膀胱反射弧的可行性。方法选择SD雄性大鼠11只，以大鼠右侧为手术侧，将其L5脊神经前、后根截断，左侧不做处理作为对照侧。术后1d3只大鼠行麦芽凝集素辣根过氧化物酶（WGA-HRP）神经示踪实验验证模型是否构建成功，应用CatWalk步态分析系统测试大鼠脊神经离断术前1d及术后3月一般性参数、个体化肢体参数、疼痛相关性参数、协调相关性参数的变化。结果神经示踪实验结果显示实验侧L5节段脊髓前、后角均未见阳性神经元及神经纤维，对照侧L5节段脊髓前角、后角均可见阳性神经元及神经纤维，从而证实模型构建成功；术后3月大鼠的一般性参数、个体化肢体参数、疼痛相关性参数等与术前1d比较差异无统计学意义（P〈0．05）．但协调相关性参数中规律性参数（RI）及右后一左前联动与术前1d相比下降，差异有统计学意义（P〈0．05）。结论离断L5脊神经后仅影响下肢行走的协调性，对其余步态相关参数无影响，因而可用于膀胱反射弧的构建。

Objective To assess the functional changes of the hind paw after SD rats being cut offthe L5 spinal nerve by CatWalk gait analysis. Methods Eleven male SD rats were chosen; the right side of these rats, as the experimental side, was cut the ventral root and dorsal root of L5 spinal nerve, while the left side was without any operation. One day after the operation, whether the models were successfully constructed was verified by tracer experiment with wheat germ agglutinin horseradish peroxidase （WGA-HRP） in 3 rats; 1 day before the operation and 3 months after the operation, the changes of such parameters as general parameters, individual paw parameters, pain-related parameters and coordination-related parameters were assessed by using CatWalk gait analysis. Results The WGA-HRP-labeled motoneurons and fibers were not found in posterior horn and anterior horn of the experimental side of L5 spinal gray matter, but both located in the left side, indicating that the models were constructed successfully. General parameters, individual paw parameters and pain-related gait parameters could be recorded 3 months after the operation, but no statistically significant difference was noted as compared with those 1 day before the operation; however, significant decrease of values of regularity index （RI） and coupling diagonal right after-left front in rats 3 months after the operation was noted as compared with those 1 day before the operation （P〈0.05）. Conclusion Only a part of coordination-related gait parameters （the lower limbs） is influenced after being cut L5 spinal nerve;therefore, L5 spinal nerve can be used for constructing of a bladder reflex arc.