摘要
离体黄瓜子叶在仅加有双蒸水的培养皿中能高频率发生不定根的现象 ,并对不定根发生过程中过氧化物酶 (POD)活性、可溶性蛋白和核酸含量的变化进行了测定。结果 ,6d苗龄子叶不定根发生率最高 ,不定根发生高峰是在培养后 3d～ 5d。在不定根发生过程中 ,过氧化物酶活性呈上升趋势 ,可溶性蛋白和核酸含量则降低。
The phenomenon was reported that adventitious roots could high frequently induced in excised cucumber cotyledons cultured with only some twice-distilled water, and the changes of activities of peroxidase(POD) and the contents of soluble protein and nucleic acid. The results showed: The highest percent of adventitious root formation occurred in cotyledons excised from 6d seedlings and the peak value of adventitious root fomation appeared within 3d～5d after culture. During adventitious root formation in excised cucumber cotyledons, the activities of POD increased whereas the contents of soluble protein and mnucleic acid decreased continuously all the time.
出处
《生物技术》
CAS
CSCD
2000年第2期3-6,共4页
Biotechnology
基金
国家自然科学基金资助项目!(3940 0 0 78)
关键词
黄瓜
不定根
子叶培养
苗龄
POD
可溶性蛋白
cucumber cotyledons
adventitious root formation
physiological changes
