烟夜蛾雄蛾性附腺因子对雌蛾性信息素合成的抑制作用

Pheromonostatic activity of male accessory gland factors in female Helicoverpa assulta

烟夜蛾Helicoverpaassulta处女蛾在交配后 1h ,其性信息素滴度即显著降低 ,72h内未见恢复。生测结果表明 ,烟夜蛾性信息素合成抑制因子主要来源于雄蛾性附腺。不同日龄雄蛾性附腺提取物的抑制活性无显著差异。光暗期对其活性具显著影响 ,暗期中雄蛾的性附腺物质对雌蛾性信息素合成具有较强抑制作用 ,而光期中雄蛾的性附腺物质不具抑制活性。在暗期的不同时间处理 ,对处女蛾性信息素合成的抑制作用无显著差异。雄蛾性附腺提取物对雌蛾性信息素合成的抑制作用与注射剂量有明显的相关性 ,0 2ME (雄蛾当量 )是产生显著抑制作用的最小剂量。对交配雌蛾注射性信息素生物合成激活神经肽 (PBAN)提取物后 ,其性信息素合成又可恢复 ,这说明雌蛾交配后 。

Significant decrease in pheromone titer of female H. assulta occurred 1 h following mating, and the pheromone titer remained low until 72 h after mating. The results of pheromonostatic bioassay showed that the pheromonostatic factors existed mainly in the male accessory gland. There was no significant difference in pheromonostatic activity among extracts prepared from accessory glands of various day age males. However the pheromonostatic activity of male accessory gland extract was affected by the phase of photoperiod, as the accessory gland extracts from the males in scotophase showed a high activity, while from those in photophase had no activity. The pheromone titers of females injected with accessory gland extracts from males at various hours in scotophase had no significant difference. There was a dose response relationship of pheromonostatic activity of male accessory gland extract, and 0 2ME (male equivalent) was the minimum dose required for effective inhibition. After injection of PBAN (Pheromone Biosynthesis Activating Nuropeptide) extract, the sex pheromone production in mated females was restored. This result suggests that the decrease of pheromone titer of mated female is due to the lack of PBAN in the sex pheromone glands.