脊椎动物肌动蛋白顺式作用元件分布的进化

Evolution of Distribution Patterns of cis-Acting Elements in 5' Regulative Regions of Vertebrate Actin

具有组织和发育表达特异性的基因，很可能具有独特的顺式作用元件的分布模式，这种模式在很大程度上决定表达的特异性。脊椎动物肌动蛋白各亚型的表达具有严格的组织特异性。为改进和完善１种顺式元件匹配预测方法，在实验资料的基础上，统计出５种顺式元件的核苷酸分布权重矩阵模式，对脊椎动物肌动蛋白基因的５’调控区进行顺式元件的匹配预测和序列分析，获得了相应亚型的特异性的顺式元件编码分布模式，并分析了其进化趋势。

Actins are expressed in a rigorous tissue-and development-manner which implies that there most probably are some unique distribution patterns of cis-acting elements in their 5' regulative regions. We improved a method to predict cis-acting element and constructed nucleotide distribution weight matrixes of 5 cis-acting elements which are concerned in vertebrate actin genes' transcription regulation. Using this method, we matched these factors in 5' regulative regions of vertebrate actin genes by comparing the sequences with these matrixes, and got six isotype-specific distribution models of these cis-acting elements. The evolutionary trends of the six models were also analyzed.