小窝蛋白-1对视网膜功能的影响

Impact of caveolin-1 on retinal function

目的:以小窝蛋白-1(Caveolin-1,Cav-1)全缺陷鼠为模型,深入研究Cav-1在视网膜中的功能。方法:通过免疫荧光及共聚焦显微镜的方法检验Cav-1在野生鼠视网膜中的表达;通过HE染色的方法检测野生型(wild type,WT)和Cav-1敲除小鼠视网膜的形态及结构;应用视网膜电流图的方法检测WT和Cav-1敲除小鼠视网膜的功能。结果:Cav-1在视网膜的多种细胞中都有表达,在Müller细胞、视网膜血管和RPE中的表达尤为强烈。视网膜电流图表明,Cav-1敲除小鼠a波与b波均较WT鼠低,但HE染色显示其视网膜形态和结构没有明显异常。结论:Cav-1缺陷小鼠表现出光感反应降低,但视网膜形态和结构均正常,提示Cav-1缺陷鼠视力下降并不源于光感受器细胞的功能缺陷,而是由于视网膜下微环境改变所致。

AIM：To investigate the role of caveolin-1（Cav-1） in the retina by examining the ocular function in Cav-1 null mice.METHODS：The expression of Cav-1 was examined by immunofluorescence combined with confocal microscopy.Retinal structure in wild type（WT） and Cav-1 null mice was examined by HE staining.In vivo retinal function in WT and Cav-1 null mice was assessed by full-field scotopic electroretinography（ERG） following overnight dark adaptation.RESULTS：Cav-1 null mice displayed reduced a-wave and b-wave amplitudes and reduced sensitivity as measured by ERG.However,the general structure of Cav-/-retina was largely normal.Collectively,this implies the impairment of retinal function in Cav-/-retina was not intrinsic to photoreceptor.CONCLUSION：The observation that Cav-1 null mice show abnormal retinal function,in vivo but normal photoreceptor structure suggests that the retinal microenvironment rather than the photoreceptor itself is impaired.These may result in a disturbance in retinal pH,water or ion homeostasis therefore altering the subretinal milieu.