摘要
介绍一种质粒DNA快速转化方法.质粒DNA加入感受态细胞,冰浴3～10min,涂布预热至37℃的平板,即可获得与常规转化方法相当的转化效率.操作步骤由5步减至2步,时间由2h减至3～10min.同时探讨了Ca2+浓度。
A rapid and simple protocol for the transformation of plasmid DNA using CaCl 2 is reported. 3～10 min incubation of cells with DNA on ice and spreading onto agar plates pre warmed to 37℃, resulting in more than 10 5 colonies/μg plasmid DNA. The effect of Ca 2+ concentration and store time at 4℃ on transformation efficiency is also discussed.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2000年第1期96-98,共3页
Progress In Biochemistry and Biophysics
